African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Extraction of uricase from Candida utilis by applying polyethylene glycol (PEG)/ NH4)2SO4 aqueous two-phase system

Yong Chen1, Yan-fa Meng2, Jin-bao Tang1*, Tong-yi Sun1, Feng-xiang Sun3 and Shu-juan Liang4*
1Department of Basic Medicine, Weifang Medical University Weifang, Shandong, 261042, China. 2Key Laboratory of Bio-resources and Eco-environment Ministry of Education, College of Life Science of Sichuan University, Chengdu 610064, China. 3Department of Common Courses, Weifang Medical University Weifang, Shandong, 261042, China. 4Department of Immunology, Key laboratory for immunology in Universities of Shanddong Province, Weifang Medical University, WeiFang, Shandong Province, 261042, People’s Republic of China.
Email: [email protected], [email protected]

  •  Accepted: 29 June 2010
  •  Published: 31 July 2010

Abstract

The purification of uricase from Candida utilis was performed by applying aqueous two-phase system (ATPS). Based on polyethylene glycols (PEG)/(NH4)2SO4ATPSs, purification fold and recovery rate were demonstrated to influence the system parameters, such as PEG molecular mass, PEG concentration, ammonium sulfate [(NH4)2SO4] concentration, inorganic concentration, pH and additional amount of crude enzyme. The ATPS formed by 25% (w/w) PEG2000, 9% (w/w) (NH4)2SO4, 2% (w/w) NaCl, pH 7.5 and addition of 5% (w/w) crude enzyme showed the best separation capability. These conditions led to the 93% (w/w) recovery of uricase with 10-fold purification.

 

Key words: Candida utilis, aqueous two-phase, extraction, purification, uricase.

Abbreviation

ATPS, Aqueous two-phase system; PEG, polyethylene glycols;BSA, bovine serum albumin.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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