African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12291

Full Length Research Paper

Molecular characterisation of canine parvovirus strains circulating in China

Q. Zhang1#, X. M. Xu2#, G. Q. Zhai1, Z. Wang1, S. H. Hou3 and H. F. Zhu3*   #These authors contributed equally to this paper.
1Collge of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.  2Jiang Su Animal Husbandry and veterinary college, Tai Zhou, 225300, China.  3Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Email: [email protected]

  •  Accepted: 09 April 2010
  •  Published: 31 July 2010

Abstract

Canine parvovirus (CPV) was first isolated at 1978 in the USA. Analysis of CPV isolates by monoclonal antibodies and restriction enzymes have shown that after the first emergence of CPV (CPV-2) it evolved to give rise to new antigenic types, which were designated CPV types 2a, type 2b and type 2c. These new types have replaced the original CPV type 2, although the proportions of each of the new antigenic types vary in different countries. In China, CPV infections were first observed in 1982, however, there has been no information concerning the antigenic types of CPV prevailing in China now. In this study, we designed a PCR assay to type canine parvovirus strains in fecal samples collected from symptomatic dogs from 2006 to 2009. Our data showed that the CPV prevalent strain is mostly 2b, the proportion of CPV-2a is very low, no CPV-2c and CPV-2 were observed.

 

Key words: Canine parvovirus; ; polymerase chain reaction; ; molecular characterization.

Abbreviation

CPV, Canine parvovirus; PCR, polymerase chain reaction; PBS,phosphate-buffered saline.