July 2010
Cytoplasmic and nuclear DNA markers as powerful tools in populations’ studies and in setting conservation strategies
Plants are distinguished among eukaryotes in possessing two DNA-containing organelles, the mitochondrion and the plastid, whereas, most eucaryotes contain only the mitochondrial genome. Recently, both organelles are used efficiently in population studies as plant geneticists developed molecular techniques that facilitated the study of plant diversity and evolution. In this paper, some comparisons among organelle and...
July 2010
Confirmation of sunflower F1 hybrids using SDS-PAGE analysis
Among bio-chemical techniques, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is most widely used due to its validity and simplicity for describing genetic structures of group of plants. In the present study, SDS-PAGE was used to confirm sunflower hybrids. Four male sterile lines, four restorer lines and their sixteen F1 hybrids were analyzed. Based on results of electrophoretic band spectra,...
July 2010
Microsatellite loci and peroxidase alleles correlation in somaclonal variation of Eucalyptus microtheca F. Muell
The aim of this study was to investigate the correlation between biochemical and molecular markers in Eucalyptus microtheca F. Muell. under in vitro culture. For this mean, twig-derived explants obtained from Eucalyptus microtheca 1-year-old seedling were cultured on modified MS medium, supplemented with different concentrations of NAA, Kin and TDZ. POD (peroxidase)...
July 2010
Effect of the growth retardant Cycocel® in controlling the growth of Dombeya burgessiae
Pink dombeya (Dombeya burgessiae) was tested for its potential as a flowering potted plant, using the growth retardant Cycocel® (2-chloroethyl)-trimethylammonium chloride. The treatments included a control, 0.5, 1, 2 and 3 mg/L of Cycocel® per pot and were applied when seedlings reached 7 - 8 cm in height. All treatments significantly reduced plant height. The plants treated with 0.5 mg/L were only...
July 2010
Development of adjustable male sterile plant in broccoli by antisense DAD1 fragment transformation
Male sterile line is very important in hybrid seed production in broccoli (Brassicaoleracea L. var. italica). A fragment of BoiDAD1F was amplified from this specie using primers designed against the conserved DAD1F fragment of DAD1 genes inArabidopsis thaliana and Brassica rapa. The BoiDAD1F sequence was 88 and 99% similar to the homologous genes...
July 2010
Study of genetic diversity in finger millet (Eleusine coracana L. Gaertn) using RAPD markers
Germplasm identification and characterization is an important link between conservation and utilization of plant genetic resources. The present study was conducted to characterize the genetic diversity using twelve germplasm of finger millet including two of the same variety (VL-149) but from different regions. Three replica of each germplasm was amplified using seventeen random primers. A total of 113 distinct...
July 2010
Molecular mapping of MS-cd1 gene in Chinese kale
A dominant male sterility (DGMS) line 79-399-3 was developed from spontaneous mutation in Brassica oleracea var. capitata and has been widely used in the production of hybrid cultivar in China. In this line, male sterility is controlled by a dominant gene Ms-cd1. In the present study, primary mapping of Ms-cd1 was conducted by screening a segregating population developed by four times...
July 2010
Molecular characterisation of canine parvovirus strains circulating in China
Canine parvovirus (CPV) was first isolated at 1978 in the USA. Analysis of CPV isolates by monoclonal antibodies and restriction enzymes have shown that after the first emergence of CPV (CPV-2) it evolved to give rise to new antigenic types, which were designated CPV types 2a, type 2b and type 2c. These new types have replaced the original CPV type 2, although the proportions of each of the new antigenic types vary in...
July 2010
Sequence comparison and phylogenetic analysis of core gene of hepatitis C virus from Pakistani population
In Pakistan, more than 10 million people are living with hepatitis C virus (HCV) with high morbidity and mortality. The aims of the present study are to report HCV core gene sequences from Pakistani population and perform their sequence comparison/phylogenetic analysis. The core gene of HCV has been cloned from six different patients and sequences submitted at the National Center of Biotechnology Information (NCBI)....
July 2010
Dewaterability of sludge digested in extended aeration plants using conventional sand drying beds
Dewaterability of unconditioned sludge digested in full scale and lab scale experiments using either extended aeration (EA) or anaerobic digestion were compared on full and lab scale sand drying beds. Sludge digested in EA plants resulted in improvement in sludge dewaterability compared to sludge digested anaerobically. This was demonstrated by comparing capillary suction time, time to filter a specific amount of water,...
July 2010
The optimization of voltage parameter for tissue electroporation in somatic embryos of Astragalus chrysochlorus (Leguminosae)
Somatic embryo tissues of Astragalus chrysochlorus were transformed with the b-glucuronidase (GUS) and neomycin phosphotransferase II (npt II) genes by electroporation. The effect of electric field strength was tested for transient expression. It was found that 1000 V/cm and 200 ms and 1 pulse was the optimum combination to transform embryo tissues (expression level...
July 2010
Effect of chromium toxicity on germination and early seedling growth in melon (Cucumis melo L.)
This study was conducted to determine and compare the inhibitory effects of chromium on seed germination and early seedling growth of melon (Cucumis meloL.). Chromium applications were controls; 2.5, 5, 10, 25, 50, 75, 100, 200 and 300 mgl-1 Cr in germination stage, and controls; 2.5, 5, 10, 20, 30, 40, 50, 60 and 70 mgl-1 Cr in early seedling stage. Excess chromium was limited to germination rate,...
July 2010
Rapid multiplication of Safed musli (Chlorophytum borivilianum) through shoot proliferation
Young shoot buds were used as explants for rapid multiplication of Safed musli (Chlorophytum borivilianum). The explants were cultured onto medium containing basal salts of Murashige and Skoog (MS) and various concentrations of 6-benzylaminopurine (BAP) and kinetin (KIN) for shoot induction. Treatment containing 3.0 mg/l BAP produced the highest mean number of shoots per explants (18.90) and a mean length of shoots...
July 2010
Increasing storability of Ceratoides arborescens seeds in ultradry storage
This research was designed to determine whether ultradry storage improves the longevity of Ceratoides arborescens seeds. Ultradry C. arborescens seeds were obtained in a desiccating container with silica gel, of which seed moisture content was from 10.87 to 3.78%, and stored at 4°C and room temperature (15 - 20°C) for 12 months. The results indicated that ultradrying treatment did...
July 2010
Water management and reuse opportunities in a thermal power plant in Jordan
The Rehab power plant located in the Northern part of Jordan is presented as a case study of industrial water management. This power plant consumes boiler feed water in the amount of 200 m3/d of the fresh ground water available from nearby wells and it produces 193 m3/d of wastewater. Fifty seven water samples were taken from the different water treatment unit's effluents to evaluate the efficiency of these...
July 2010
Isolation and screening of actinomycetes from Sundarbans soil for antibacterial activity
A total of 55 actinomycetes isolates from soil sample of Karanjal region in Sundarbans were characterized for morphological identification and antimicrobial activity. Four general such as Actinomyces, Nocardia, Streptomyces andMicromonospora with total numbers of isolates were 27, 14, 11 and 3, respectively, were identified from the sample. Twenty actinomycetes isolates produced antibiotic...
July 2010
Light trap surveys for moths in Sile region of Istanbul, Turkey
In this study, Heterocera species collected by light trap method in Sile region, Istanbul province, Turkey during the years of 2007 - 2008 was evaluated. A total of 194 specimens were collected from 48 different locations in Sile. According to identification results, 70 species belonging to 15 families were recorded. The family Noctuidae was represented by the highest number of species (26), followed by Geometridae (16)...
July 2010
Growth performances, carcasses parameters and meat fatty acid composition of lamb fed green oak acorns (Quercus ilex) based diet
The aim of this experiment is to compare the effects of diets containing green oak acorns (GO) and barley (BL) on the growth performances, carcasses parameters and the composition in fatty acids of lambs. Two groups of five lambs each were fed, respectively, during 105 days with diets containing 50% of oaks acorns and 50% of barley. At the end of the test, the animals of the two diets expressed comparable body...
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