African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12209

Full Length Research Paper

Protamine 3 expressions in crossbred bull spermatozoa may not be a prognostic marker for differentiating good and poor quality semen

Sushil Kumar
  • Sushil Kumar
  • Molecular Genetics Laboratory, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh-250001, India.
  • Google Scholar
Umesh Singh
  • Umesh Singh
  • Molecular Genetics Laboratory, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh-250001, India.
  • Google Scholar
Indrajit Ganguly
  • Indrajit Ganguly
  • Molecular Genetics Laboratory, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh-250001, India.
  • Google Scholar
Rajib Deb*
  • Rajib Deb*
  • Molecular Genetics Laboratory, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh-250001, India.
  • Google Scholar
Rani Singh
  • Rani Singh
  • Molecular Genetics Laboratory, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh-250001, India.
  • Google Scholar
Sandeep Mann
  • Sandeep Mann
  • Molecular Genetics Laboratory, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh-250001, India.
  • Google Scholar
G. Sengar
  • G. Sengar
  • Molecular Genetics Laboratory, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh-250001, India.
  • Google Scholar
D. K. Mandal
  • D. K. Mandal
  • Molecular Genetics Laboratory, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh-250001, India.
  • Google Scholar
Mahesh Kumar
  • Mahesh Kumar
  • Molecular Genetics Laboratory, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh-250001, India.
  • Google Scholar
Arjava Sharma
  • Arjava Sharma
  • Molecular Genetics Laboratory, Project Directorate on Cattle (ICAR), Meerut, Uttar Pradesh-250001, India.
  • Google Scholar


  •  Received: 04 December 2013
  •  Accepted: 06 May 2014
  •  Published: 14 May 2014

Abstract

Protamines are short and highly basic sperm-specific nuclear proteins. Though the expression profile of protamines 1 and 2 is well known, but there is little knowledge of protamine 3 expression in bovine semen. In this study, Frieswal (HF x Sahiwal) crossbred bulls were categorized into two groups (good and poor quality) based on the initial progressive motility of semen and other seminal parameters. The mRNA expression of PRM3 gene among two groups was evaluated by real time quantitative polymerase chain reaction (PCR) using TaqMan chemistry, where peptidylprolyl isomerase A (PPIA) was used as an internal control. Our finding revealed that expression of PRM3 was down regulated in poor quality semen producers as compared to good quality semen producing group, but unfortunately no significant difference of transcript abundance was observed between the groups. To shed light on present findings, it can thus assume that PRM3 may not be a prognostic marker to differentiate good and poor quality bull semen in Frieswal cattle.

 

Key words: Protamine 3, Frieswal, semen, expression.