African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12198

Table of Content: 14 May, 2014; 13(20)

May 2014

Controlling water deficit by osmolytes and enzymes: Enhancement of carbohydrate mobilization to overcome osmotic stress in wheat subjected to water deficit conditions

The present study demonstrates the effect of polyethylene glycol-6000 (PEG) induced water-deficit stress on growth, physiological and biochemical responses in six wheat (Triticum aestivum L.) cultivars viz. C 306, C 273, PBW 175 (drought tolerant), PBW 534, PBW 343 and PBW 550 (drought susceptibile) at seedling stage for six days. Stress caused short stature in shoot and elongated roots in all cultivars. Significant...

Author(s): Asthir, B., Gulati, A. and Bains, N. S.

May 2014

Application of bioinformatics to optimization of serum proteome in oral leukoplakia and oral squamous cell carcinoma

Intervention to the evolution and metastasis of oral squamous cell carcinoma (OSCC) from oral leukoplakia (OLK) out of normal mucosa is currently far from ultimate. New technologies and insights are reckoned on for etiology and clinical protocol of OSCC and mucosa lesions. SELDI-TOF-MS technology, Support vector machine, Discriminate Analysis, and CM10 protein chip were applied to study the sera proteomes of 32 healthy...

Author(s): Hong He, Gang Sun, Feiyun Ping, Zhijian Xie, Huiming Wang, Yining Li and Kai Zhang

May 2014

Geochemical aspects of Meretrix casta (bivalve) shells of Vellar estuary, southeast coast of India

The bivalve mollusk, Meretrix casta shells are abundant in the Vellar estuary along the East coast of India, they are economically important and used as chief raw material for many lime based industries. Their shells are harvested in large quantities for meat and lime production. The present study focused on understanding the chemical characteristics of M. casta shells, collected at eight different locations in the...

Author(s): Shoba, K. and Senthilkumar, G. R

May 2014

Protamine 3 expressions in crossbred bull spermatozoa may not be a prognostic marker for differentiating good and poor quality semen

Protamines are short and highly basic sperm-specific nuclear proteins. Though the expression profile of protamines 1 and 2 is well known, but there is little knowledge of protamine 3 expression in bovine semen. In this study, Frieswal (HF x Sahiwal) crossbred bulls were categorized into two groups (good and poor quality) based on the initial progressive motility of semen and other seminal parameters. The mRNA expression...

Author(s): Sushil Kumar, Umesh Singh, Indrajit Ganguly#, Rajib Deb, Rani Singh, Sandeep Mann, G. Sengar, D. K. Mandal, Mahesh Kumar and Arjava Sharma

May 2014

Genetic variability assessment of accessions of Corchorus olitorius L. using sodium dodecyl sulphate polyacrylamide gel electrophoresis

Corchorus olitorius is one of the neglected indigenous leafy vegetables in Nigeria. Majority of the accessions of this species stored at the National Centre for Genetic Resources and Biotechnology (NACGRAB) Moor Plantation, Ibadan, Nigeria, have only been characterized morphologically. To provide further information on the extent of genetic diversity, this study was initiated to assess the genetic variabilities among 14...

Author(s): Chinyere Chioma Isuosuo and Florence Ifeoma Akaneme

May 2014

Analysis association of milk fat and protein percent in quantitative trait locus (QTLs) on chromosomes 1, 6, 7 and 20 in Iranian Holstein cattle using ten micro satellite markers

Protein and fat percent as content of milk are high-priority criteria for financial aims and selection of programs in dairy cattle. In order to incorporate information on marker- quantitative trait locus (QTL) associations, QTL detection and parameter estimation should also be performed. Microsatellite markers are most valuable markers in association studies between different loci with the production traits. With the...

Author(s): A. Attar, G. Rahimi, M. R. Nassiri and B. Sadeghi

May 2014

Effect of growth regulators and explant types on callus induction in Telfairia occidentalis Hook F

Different concentrations of growth regulators and three types of explants were investigated for their efficiency on callus induction in Telfairia occidentalis with a view of providing baseline information for the development of a callus initiation protocol. Three concentrations of kinetin (KN) (0.1, 3.3 and 5.0 mg/L) in combination with two concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D) (2.0 and 5.0 mg/L) and...

Author(s): Sakpere, A.M. A., Ajayi, S. A and Adelusi, A.A.

May 2014

Morpho-physiological characterization of Indian wheat genotypes and their evaluation under drought condition

To study the morpho-physiological and genetic diversity analysis of Indian wheat cultivars a total of 10 genotypes were collected namely: HD-2133, HUW-825, R-54, K-9533, V-110, V-70, HUW-213, V-23, VWTH-08-07 and HUW-37 to evaluate the genotypes under drought stress the seeds of 10 varieties were treated with polyethylene glycol (PEG) for inducing stress condition. Among the morphological characteristics of wheat...

Author(s): Naresh Pratap Singh, Pramod Kumar Pal and Sandeep Kumar Vaishali

May 2014

Cloning and tissue expression of cytochrome P450 1B1 and 1C1 genes from Javanese Medaka, Oryzias Javanicus, under environmental stress conditions

Cytochrome P450 1 (CYP1) is widely used as an indicator of exposure to environmental contaminants. In the study, two full-length complementary DNAs encode for CYP1B1 and CYP1C1 were cloned from medaka liver exposed to 500 ppb β-naphthoflavone for 24 h. CYP1B1, having 1984 bp, contains an open reading frame of 1551 bp encoding a protein of 517 amino acids; while, CYP1C1 having 2601 bp consists of an open reading...

Author(s): Trieu Tuan, Yoshino Kaminishi, Aki Funahashi, Abeer A. I. Hassanin and Takao Itakura

May 2014

Effect of extracts of Trichilia silvatica C. DC., on development and reproduction parameters of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)

The objective of this study was to evaluate the effects of the methanolic extracts from the leaves, bark and flowers of Trichilia silvatica on Spodoptera frugiperda. Also, it was use in evaluating the total phenolic and flavonoid content of methanolic extracts. We also reported chemical study on the most active extract. Corn leaves were immersed in a 1% methanolic extract solution and fed to second instars of S....

Author(s): Alessandra F. Freitas, Anelise S. N. Formagio, Fabricio F. Pereira, Jéssica T. Lucchetta and Maria Do Carmo Vieira

May 2014

Physicochemical properties of lignocellulosic biofibres from South Eastern Nigeria: Their suitability for biocomposite technology

Five plant raw materials collected from South Eastern part of Nigeria were used for biofibre extraction and analysis to assess their suitability for biocomposite production. Lignocellulosic biofibres were extracted from young stems of Adenia lobata, Ampelocissus leonensis, Cissus palmatifida, Morinda morindoides and Urena lobata through natural water retting process for a period of 14 - 16 days and the resulting fibres...

Author(s): Agu, Chidozie Victor, , , Njoku, O. U., Chilaka, F. C., Agbiogwu, D, Iloabuchi, K. V. and Ukazu, B.

May 2014

Changes in protein solubility, fermentative capacity, viscoelasticity and breadmaking of frozen dough

The use of frozen dough remedied availability of fresh bread. However, bread elaborated from frozen dough has less volume and texture is firmer. This study evaluates how storage affects the protein solubility, fermentative capacity and viscoelasticity of frozen dough. In addition to examining the effects of storage on the quality of the final baked bread. Dough was frozen at a rate of -0.146°C/min and stored at...

Author(s): Elisa Magaña-Barajas, Benjamín Ramírez-Wong, Patricia Isabel Torres-Chavez, Dalia Isabel Sánchez-Machado and Jaime López-Cervantes