African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

Full Length Research Paper

Genetic diversity in Kenyan populations of Acacia senegal (L.) willd revealed by combined RAPD and ISSR markers

Chiveu Chemulanga Josiah1*, Dangasuk Otto George2, Omunyin Michael Eleazar1 and Wachira Francis Nyamu3
1Department of Seed, Crop and Horticultural Sciences, Moi University, P.O. Box 1125, Eldoret, Kenya. 2Department of Biological Sciences, Moi University, P.O. Box 1125, Eldoret, Kenya. 3Department of Biochemistry and Molecular Biology, Egerton University, P.O. Box 1577, Nakuru, Kenya.
Email: [email protected]

  •  Accepted: 11 June 2008
  •  Published: 18 July 2008

Abstract

Acacia senegal belongs to the subgenus, Aculeiferum. It is an African arid and semi arid zone multipurpose tree species, highly valued for gum arabic production, agroforestry and desertification control besides  other multiple uses. Genetic variation and resulting variable groupings were assessed using combined RAPD+ISSR markers within and among four Kenyan populations of A. senegal. Using 10 RAPD and 5 ISSR primers, a total of 55 polymorphic bands with an average of 3.6 polymorphic loci per RAPD+ISSR primer were detected. The mean Nei’s gene diversity index (H) for the populations was 0.283 and mean observed number of alleles per locus (A) was 1.982. Much of the genetic variation resided within the populations based on the Analysis of Molecular Variance (AMOVA) (86%). The dendrogram derived using the Unweighted Pair-Group Method with Arithmetic averaging (UPGMA) clustered the Garissa and Wajir populations in one group and the Samburu and Baringo populations in the other, reflecting geographical sub-structuring of the genetic diversity. It was therefore recommended that selection of desired important economic traits for improvement and conservation should target individual trees within populations and cover the entire ecological amplitude of the populations.

 

Key words: Acacia senegal, genetic diversity, combined RAPD+ISSR markers, multipurpose, Kenya, selection, conservation.