African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Temperature effect on dextransucrase production by Leuconostoc mesenteroides FT 045 B isolated from Alcohol and Sugar Mill Plant

Mariana Cortezi1, Rubens Monti2 and Jonas Contiero1*
  1 Departamento de Bioquímica e Microbiologia, Instituto de Biociências de Rio Claro/ UNESP, São Paulo, SP, Brasil. 2Departamento de Alimentos e Nutrição, Faculdade de Ciências Farmacêuticas UNESP, Araraquara, São Paulo, SP, Brasil.
Email: [email protected]

  •  Accepted: 13 August 2004
  •  Published: 31 March 2005

Abstract

 

Temperature (23 to 31°C) and sucrose concentration (3 and 4%) effects on dextransucrase production by Leuconostoc mesenteroides NRRL B 512 (F) andLeuconostoc mesenteroides FT 045 B were studied. The conditions in all fermentations were:  total reaction volume 2 L, 132 rev. min-1, 0.5 vvm and pH 6.0. The optimum temperature for enzyme yield for strain NRRL B 512 (F) was 23°C, where at 8-h fermentation was possible to achieve 49.3 DSU/mL. When FT 045 B strain was utilized, 3.2 DSU/mL was obtained at temperature 23 to 25 °C.

 

Key words: Dextran, dextransucrase, Leuconostoc mesenteroides NRRL B512 (F), Leuconostoc mesenteroides FT045 B, temperature, enzyme activity.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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