African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Microsatellite based investigation of genetic diversity in 24 synthetic wheat cultivars

Muhammad Shakeel1 and Sadiq Azam2*
1Department of Biotechnology, Bacha Khan University, Palosa Campus Charsadda, Pakistan. 2Centre for Biotechnology and Microbiology, University of Peshawar, Khyber Pakhtunkhwa, Pakistan.  
Email: [email protected]

  •  Accepted: 23 May 2012
  •  Published: 06 September 2012

Abstract

Common bread wheat has very low genetic diversity. Plant breeders find it difficult to get appropriate germplasm which can be used for bread wheat improvement. Wild plant species is one of the potential sources for conferring resistant genes which can be exploited for bread wheat improvement. The challenge is to utilize these wild relatives into existing food crops without losing the genetic diversity. This study aimed to monitor genetic variability in the synthetic hexaploid wheats using microsatellites molecular markers. The polymorphism revealed by microsatellites was not very prevalent; however more Simple Sequence Repeat (SSR) need to be used to select diverse synthetic lines which can be crossed with common bread wheat to enhance the crop yield and resistance against various stresses.

 

Key words: Microsatellite, germplasms, hexaploids, polymerase chain reaction, wheat.

Abbreviation

SSR, Simple sequence repeat; SH, synthetic hexaploids.