African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12072

Full Length Research Paper

Effects of ethanolic extract of Waltheria indica aerial parts on some liver and kidney function indices in albino rats

Hamidu J. L.
  • Hamidu J. L.
  • of Human Physiology, College of Medical Sciences, University of Maiduguri, Maiduguri, Borno State, Nigeria.
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Ayo J. O.
  • Ayo J. O.
  • Department of Veterinary Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
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Adelaiye A. B.
  • Adelaiye A. B.
  • Department of Human Physiology, Faculty of Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
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Abubakar M. S.
  • Abubakar M. S.
  • Department of Pharmacognosy and Drug Development, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
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  •  Received: 03 March 2017
  •  Accepted: 11 October 2017
  •  Published: 09 May 2018

Abstract

This study evaluated the toxicological effects of chronic treatment with ethanolic extract of Waltheria indica aerial portion on body weight, hematological and biochemical parameters in albino rats using standard methods. Rats treated with 400 mg/kg body weight (bw)/day of the extract showed no behavioural changes. However, there was general reduction of activity in rats given 800 and 1,600 mg/kg bw/day of the extract. Also, the LD50 treated rats exhibited hypoactivity, grooming, prostration and irritation during treatment in the third and fourth weeks of treatment period. The data on body weight changes indicated that there were no significant differences in body weight between the control group and groups that received different doses of the extract (p<0.50). Haematological results for the red blood cell, haemoglobin, packed cell volume, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration in extract treated rats showed no significant changes at all doses of treatments when compared with controls in female rats. However, data on mean corpuscular haemoglobin in the male rats treated with 1,600 mg/kg showed a significantly decreased value when compared with controls. On the other hands, white blood cell counts decreased significantly after treatment with 400 and 800 mg/kg bw/day extract in the male rats. Lymphocytes count was also decreased significantly in males treated with 800 mg/kg bw/day of extract. Alanine transaminase (ALT), total bilirubin and creatinine increased significantly, respectively at 800 and 1,600 mg/kg bw/day doses of extract when compared with the control rats (p<0.05). In conclusion, the overall data of this study suggest that the oral administration of W. indica extract did not induce any toxic effects, especially when administered at low doses; however, further investigation is needed to evaluate its chronic toxicity.

Key words: Waltheria indica, Wistar rats, evaluation, toxicological, biochemical, haematological, parameters.

 

Abbreviation

ALT, Alanine transaminase; AST, aspartate transaminase; DLC, differential leucocyte count; Hb, haemoglobin; MCV, mean corpuscular volume; MCH, mean corpuscular haemoglobin; MCHC, mean corpuscular haemoglobin concentration; PVC, packed cell volume; RBC, red blood cell; WBC, white blood cell.