African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12254

Full Length Research Paper

Comparison of phenolic and volatile profiles of edible and toxic forms of Detarium senegalense J. F. GMEL

Nafissatou DIOP NDIAYE
  • Nafissatou DIOP NDIAYE
  • Institut de Technologie Alimentaire, Route des Pères Maristes, B.P. 2765, Dakar - Hann, Sénégal.
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Sylvie MUNIER
  • Sylvie MUNIER
  • Université Montpellier I, UFR Pharmacie, UMR95 QualiSud, 15 av. Charles Flahault, F-34090 Montpellier, France.
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Yves PELISSIER
  • Yves PELISSIER
  • Université Montpellier I, UFR Pharmacie, UMR95 QualiSud, 15 av. Charles Flahault, F-34090 Montpellier, France.
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Frédéric BOUDARD
  • Frédéric BOUDARD
  • Université Montpellier I, UFR Pharmacie, UMR95 QualiSud, 15 av. Charles Flahault, F-34090 Montpellier, France.
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Christian MERTZ
  • Christian MERTZ
  • Cirad, UMR95 QualiSud, 73 rue J.F. Breton, TA B-95/16, F-34398 Montpellier cedex 5, France.
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Marc LEBRUN
  • Marc LEBRUN
  • Cirad, UMR95 QualiSud, 73 rue J.F. Breton, TA B-95/16, F-34398 Montpellier cedex 5, France.
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Claudie DHUIQUE-MAYER
  • Claudie DHUIQUE-MAYER
  • Cirad, UMR95 QualiSud, 73 rue J.F. Breton, TA B-95/16, F-34398 Montpellier cedex 5, France.
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Manuel DORNIER
  • Manuel DORNIER
  • Montpellier SupAgro, UMR95 QualiSud, 1101 av. Agropolis, B.P. 5098, F-34093 Montpellier cedex 5, France.
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  •  Received: 01 December 2015
  •  Accepted: 21 March 2016
  •  Published: 20 April 2016

Abstract

In Senegal, Detarium senegalense J.F. Gmel. (ditax in Wolof) is one of the most important ¶important forest fruit species. ¶However, exploitation of the edible fruit is based on local people’s knowledge. Only trees whose fruits are consumed by animals are exploited. ¶To identify them, a chemical comparison of edible and toxic forms was done in order to highlight differences between both forms. Dichloromethane leaf extracts from toxic and edible trees were analyzed by gas chromatography. Phenolic profile and volatile compounds from fruits extracts were studied respectively by High Performance Liquid Chromatography-mass spectrometry (HPLC-MS) and Gas Chromatography Mass Spectrometry (GC-MS). Cytotoxicity effect of fruits extracts was evaluated on murine macrophage cells J774 A1. GC-analysis of dichloromethane leaf extracts revealed the presence of lupenone and lupeol only in toxic extracts. 6’-O-galloyl-epiheterodendrin and isovaleronitrile were detected in toxic pulp. However, no cytotoxic effect was found in our conditions. This study has given the opportunity to identify within the same species, compounds which could differentiate both edible and toxic forms. Nevertheless further studies are needed to better understand which compounds are responsible for toxicity in the toxic form.

Key words: Ditax, Detarium senegalense, toxicity, lupeol, lupenone, cyanogenic glycoside, isovaleronitrile.