African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Production of polyploids from cultured shoot tips of Eucalyptus globulus Labill by treatment with colchicine

H. Lin1,2, M. Jian2, L. Y. Liang2, W. J. Pei2, X. Z. Liu1 and H. Y. Zhang1*
1Key Laboratory of Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, Yunnan Province-650224, P.R. China. 2Leizhou Forestry Bureau, Shuiqi, Guangdong Province-524348, P.R. China.
Email: [email protected]

  •  Accepted: 12 March 2010
  •  Published: 30 April 2010

Abstract

Polyploids from cultured shoot tips of Eucalyptus globulus were produced by treatment with colchicine. Results showed that the combination of 0.5% colchicine and treating multiple shoot clumps for 4 days was the most appropriate conditions for E. globulus polyploidy induction and the effect of the use of multiple shoot clumps for colchicine polyploidy-induced was better than single buds. By comparing the polyploidy plants with normal diploid ones in morphology, leaves of polyploid plants were thicker, larger, and darker green. The chromosome number of polyploidy plants that had been identified in morphology was 2n = 4x = 44, while that of diploids was 2n = 2x = 22.

 

Key words: Eucalyptus globulus, polyploids, cultured shoot, colchicine.

Abbreviation

IAA, Indole-3-acetic acid; DMSO, dimethyl sulphoxide; MS,Murashige and Skoog; NAA, α-naphthalene acetic acid; IBA, indole-3-butyric acid;BA, 6-benzylaminopurine.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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