African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

The kinetics of glucose production from rice straw by Aspergillus niger

B. O. Aderemi1*, E. Abu2 and B. K. Highina3
  1Department of Chemical Engineering, Ahmadu Bello University, Zaria, Nigeria. 2Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria. 3Department of Chemical Engineering, University of Maiduguri, Maiduguri, Nigeria.
Email: [email protected]

  •  Accepted: 02 May 2008
  •  Published: 03 June 2008

Abstract

 

In this investigation, glucose was produced from rice straw using cells ofAspergillus niger, isolated from maize grain. Glucose yield was found to increase from 43 to 87% as the rice straw particle size decreased from 425 to 75 µm, while the optimal temperature and pH were found within the range of 45 - 50°C and 4.5 - 5 respectively. The concentration and rate of glucose production was observed to depend on pretreatment of rice straw, substrate concentration and cell loading.A kinetic model rate expression has been developed for such a process based on the Michaelis – Mentens and Lineweaver – Burk approach. Comparison between the experimental data and those predicted from the rate model indicate good agreement with a mean absolute deviation of about 0.2.

 

Key words: Rice straw, cellulose, glucose, Aspergillus niger, enzyme loading, kinetic parameters, kinetic model.