African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

In vitro rapid and mass multiplication of highly valuable medicinal plant Bacopa monnieri (L.) Wettst.

  Sudhir Sharma1*, Barkha Kamal2, Neelima Rathi1, Sudhir Chauhan1, Vikas Jadon2, Neha Vats1, Ashok Gehlot3 and Sarita Arya3  
  1Tissue Culture Discipline, Botany Division, Forest Research Institute, Dehradun (Uttarakhand), India. 2Plant molecular Biology Laboratory, Department of Biotechnology, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences and Research, Balawala, Dehradun (Uttarakhand), India. 3Forest Genetics and Tree Breeding Division, Arid Forest Research Institute, Jodhpur (Rajasthan), India.  
Email: [email protected]

  •  Accepted: 24 August 2010
  •  Published: 06 December 2010

Abstract

 

A protocol has been developed for micropropagation of Bacopa monnieri (L) Wettst, a medicinal plant of high commercial potential with legendary reputation as a memory vitalizer. Nodal segments containing axillary buds were surface sterilized with 0.1% solution of mercuric chloride for 5 min and were inoculated aseptically on culture medium, axillary bud break was achieved in 100% of cultures in semisolid MS medium supplemented with 0.2 mg/l BAP. These proliferated in vitro axillary shoots were excised and cut into groups of shoot clusters and subcultured on MS medium supplemented with 0.2 mg/l BAP for shoot multiplication. 100% in vitrorooting was obtained when shoot clusters were cultured on MS medium supplemented with 0.15 mg/l IBA. The rooted plantlets were hardened, acclimatized and successfully established in field.

 

Key words: Bacopa monnieri, nodal segments, micropropagation, plant growth regulators.