African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12291

Full Length Research Paper

Improving peanut protein quality: Expression of a synthetic storage protein

N’Nan Affoué Sylvie DIBY
  • N’Nan Affoué Sylvie DIBY
  • Department of Food and Animal Sciences, Alabama A and M University, P. O. Box 1628, Normal AL 35762, USA.
  • Google Scholar
Koffi N’Da KONAN
  • Koffi N’Da KONAN
  • Department of Food and Animal Sciences, Alabama A and M University, P. O. Box 1628, Normal AL 35762, USA.
  • Google Scholar
Anthony Okello ANANGA
  • Anthony Okello ANANGA
  • Department of Food and Animal Sciences, Alabama A and M University, P. O. Box 1628, Normal AL 35762, USA.
  • Google Scholar
Hortense DODO
  • Hortense DODO
  • Department of Food and Animal Sciences, Alabama A and M University, P. O. Box 1628, Normal AL 35762, USA.
  • Google Scholar


  •  Received: 12 November 2019
  •  Accepted: 31 January 2020
  •  Published: 31 May 2020

Abstract

Peanut is an affordable legume used in most households. It represents one of the most important protein supplies worldwide. However, peanut proteins are deficient in several essential amino acids (EAA), like most plant proteins; whereas plants are the main source of dietary proteins consumed by humans and livestock. This could lead to protein malnutrition in areas where people diet relies on one or two staple foods. Based on its high nutritional value, peanut is a good candidate for genetic biofortification. This study is aimed at expressing an EAA-rich artificial storage protein (ASPx) into peanut seeds for increased nutritive value. The ASPx derived gene was introduced into peanut via Agrobacterium-mediated transformation. Molecular analysis of regenerated kanamycin resistant plants using polymerase chain reaction (PCR) and Southern hybridization indicate the stable integration of one copy ASPx gene in transgenic plants. The expression of the ASPx in transgenic peanuts seeds was detected by mass spectrometry (multiple reaction monitoring). Amino acids analysis showed an increase of 12 to 19% of most EAA (Val, Tyr, Phe, Iso, Leu, Met) in a transgenic line. The results show that the nutritional quality of peanut could be improved.

 

Key words: Genetic biofortification, essential amino acid, storage protein, peanut.