African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

A plasma membrane H+ATPase gene is germination-induced in wheat embryos

Mahmut Caliskan1*, Stavros Bashiardes2 and Andrew C. Cuming2
  1Mustafa Kemal University, Biology Dept. 31024  Hatay-Turkey. 2University of Leeds, Department of Genetics LS2 9JT Leeds-England.
Email: [email protected]

  •  Accepted: 15 May 2009
  •  Published: 18 January 2010

Abstract

 

The expression pattern of a germination specific plasma membrane H+-ATPase was analyzed by RT-PCR and in situ RNA hybridization methods. RT-PCR results revealed that germination specific plasma membrane H+-ATPase accumulation was detectable in all organs and tissues of germinating wheat embryos. H+-ATPase expression was not observed in dry wheat embryos and in immature wheat embryos. In situ RNA hybridization indicated that germination specific plasma membrane H+-ATPase gene expression was associated with all organs of germinating wheat tissues. The accumulation of H+-ATPase mRNA was more heavily on the cells of vascular bundles and epidermal cells of coleoptiles. Since germination specific plasma membrane H+-ATPase gene was identified as a growth related gene, interest was focused on the activity of growth regulators (GA, IAA, ABA) and stress factors, NaCl and Mannitol, on H+-ATPase gene expression. The results indicated that there were not any dramatic changes in the accumulation of germination specific plasma membrane H+-ATPase gene in any case. More rigorous analysis is necessary to evaluate the effect of growth regulators on germination specific plasma membrane H+-ATPase.

 

Key words: Biosynthesis, cereals, DNA, enzymes, physiology, plant cell culture.