African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12463

Full Length Research Paper

In vitro anthelmintic effect of two medicinal plants (Anogeissus leiocarpus and Daniellia oliveri) on Haemonchus contortus, an abosomal nematode of sheep in Burkina Faso

Kabore Adama1*, Belem A. M. Gaston2, Tamboura Hamidou H.1, Traore Amadou1 and Sawadogo Laya3
1Institut de l’Environnement et de Recherches Agricoles (INERA)/Département Productions Animales (DPA), 04 BP 8645 Ouagadougou/Burkina Faso. 2Institut du Développement Rural (IDR)/Université Polytechnique de Bobo-Dioulasso, 01 BP 3770 Ouagadougou 01, Burkina Faso. 3Université de Ouagadougou / UFR-SVT, 03 BP 7021 Ouagadougou 03, Burkina Faso.  
Email: [email protected]

  •  Accepted: 12 May 2009
  •  Published: 15 September 2009


A study was conducted to evaluate Anogeissus leiocarpus leaf and Daniellia oliveristem barks as effective remedy for gastrointestinal parasites. The anthelmintic activity of these extracts on eggs, first stage larvae and adults of Haemonchus contortus was examined by in vitro tests. The extracts were prepared to obtain six increasing concentrations. This was done with Phosphate Buffered Saline (PBS) for egg hatch, embryonated egg assays (75, 150, 300, 600, 1 200 and 2 400 µg/ml) and adult inhibition of motility assay (0.25, 0.5, 1, 2, 4 and 8 mg/ml). PBS and levamisole (at 0.125 µg/ml in PBS) were used as negative and positive control groups, respectively. Both plant extracts induced anthelmintic effects on the three life-cycle stages of H. contortus and these effects were significantly different when they were compared to the negative control group (PBS) (P<0.05). The effect was dose-dependent on egg hatching and first stage larvae (L1) but not on adult worms. Magnitude of effect was proportional to concentration of plant extracts for egg hatching and L1 but not for adult worms. Besides, the results showed that the D. oliveri stem bark extract was more ovicidal and larvicidal than A. leiocarpus leaves. It is concluded that these two plants do really possess anthelmintic properties.


Key words: Anthelmintic, leaf, stem bark, extracts, Anogeissus leiocarpus,Daniellia oliveriHaemonchus contortus.