African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Influence of nitrogen source and sucrose concentration on inulinase production by Kluyveromyces marxianus in fed-batch fermentation

Cazetta, M. L.1,2* and Contiero, J.1
  1Departamento de Bioquímica e Microbiologia, Instituto de Biociências, Universidade Estadual Paulista (UNESP)-São Paulo-Brasil, Av. 24A, 1515, CEP13506-900, Rio Claro, São Paulo, Brasil. 2Centro de Ciências Exatas e Tecnológicas, Universidade Federal do Recôncavo da Bahia (UFRB), Rua Rui Barbosa, 710, Campus de Cruz das Almas, CEP:44380-000, Cruz das Almas, Bahia-Brasil.
Email: [email protected]

  •  Accepted: 22 July 2011
  •  Published: 26 September 2011

Abstract

 

This work studied the influence of nitrogen source and sucrose concentration in the feeding medium for biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus. The results show that the best nitrogen source was a combination of 5 g/L of yeast extract and 10 g/L of peptone. Both cellular growth and enzymatic activity increased with sucrose concentration in the feeding medium (from 200 to 500 g/L). When the sucrose concentration reached 600 g/L, both cellular growth and enzymatic activity decreased.

 

Key words: Kluyveromyces marxianus, nitrogen, sucrose, inulinase, fed-batch fermentation.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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