African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Optimization of extraction process for ustiloxins A and B from rice false smut balls using an orthogonal array design

Tijiang Shan
  • Tijiang Shan
  • MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
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Xiaohan Wang
  • Xiaohan Wang
  • MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
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Xiaoxiang Fu
  • Xiaoxiang Fu
  • MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
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Jiajia Meng
  • Jiajia Meng
  • MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
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Weibo Sun
  • Weibo Sun
  • MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
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Ali Wang
  • Ali Wang
  • MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
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Yang Liu
  • Yang Liu
  • Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
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Ligang Zhou*
  • Ligang Zhou*
  • MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
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  •  Received: 02 August 2013
  •  Accepted: 12 September 2014
  •  Published: 17 September 2014

Abstract

Optimization of four factors, namely methanol concentration (A), extraction pH value (B), material-to-solvent ratio (C) and extraction times (D), for extraction of ustiloxins A and B from rice false smut balls was achieved by using an L18 orthogonal array design with three levels and four factors. The results show that the optimum conditions for ustiloxins A and B extraction should be A1B2C2D3 corresponding to methanol concentration at 10%, extraction pH value at 6, material-to-solvent ratio at 1:30 (g/mL) and extraction times as 3, respectively. Under the optimum extraction condition, the content of ustiloxins A and B in rice false smut balls was analyzed to be 0.80 and 0.57 mg/g, respectively, on a dry weight basis.

 

Key words: Rice false smut balls, Villosiclava virens, Ustilaginoidea virens, ustiloxins A and B, orthogonal array design, extraction optimization.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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