African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12182

Table of Content: 17 September, 2014; 13(38)

September 2014

Cryoconservation of plant germplasm native to Brazil

The preservation of biological material at -196°C, that is, at liquid nitrogen temperature, or its vapor (between -150 and -178°C), is a long-term storage procedure called cryopreservation or cryoconservation. This article reports studies made in Brazil for cryoconservation of native plant species and highlights what might be the gap in this area of germplasm conservation. Most studies use seeds as plant...

Author(s): Laila Mandel Civatti, Maria Nazaré Guimarães Marchi, Gabriela Torres-Silva, José Geraldo Aquino de Assis and Moema Cortizo Bellintani

September 2014

Analysis of eleven Y-chromosomal STR markers in Middle and South of Iraq

Samples of 320 random healthy unrelated Iraqi male persons from the Arab ethic group of Iraqi population were collected for Y chromosomal STRs typing. FTA® Technology was utilized to extract DNA from blood collected on FTA™ paper. We analyzed 11 Y chromosomal STR loci for evaluating allele frequencies and gene diversity for each Y-STR locus of the Y-Plex™ PCR amplification kit. The 11 loci include...

Author(s): Imad, Hadi Hameed , Muhanned, Abdulhasan Kareem, Aamera, Jaber Ommer and Cheah, Yoke

September 2014

Micropropagation of Jatropha curcas superior genotypes and evaluation of clonal fidelity by target region amplification polymorphism (TRAP) molecular marker and flow cytometry

The major limitation in large-scale cultivation of Jatropha curcas for use as energy crop is the inconsistent and unstable seed yield due to the heterozygous nature of the plant. A reliable in vitro regeneration system is necessary for continuous supply of quality planting material at large-scale. In this study, the interaction between the season collection of explants and capacity of in vitro regeneration shoots from...

Author(s): Mariana Crotti Franco, Daniela de Argollo Marques, Walter José Siqueira and Rodrigo Rocha Latado

September 2014

Abnormal mitosis in root meristem cells of Allium cepa L. induced by a fabric dye reactive turquoise blue (Procion MX)

Reactive Turquoise Blue (Procion MX) is a fabric dye used in small scale cotton fabric industries in various parts of India. The impact of this chemical on human health in the surrounding areas that discharge effluents is of serious concern. This needs to be assessed for short and long term effect on human genome. This investigation was aimed to find mitotic abnormalities as cytological evidence induced by the dye in...

Author(s): Subodh Kumar Tripathy and Shraddheya Patel

September 2014

In vitro propagation of garlic (Allium sativum L.) through adventitious shoot organogenesis

The present study investigates in vitro regeneration of three garlic cultivars; that is, Balady, Sids 40 and VFG 180 (3 - 1) as well as a garlic wild type through adventitious shoot organogenesis. Shoot and root apices were subjected to eight callus induction treatments. A combination of 1 mg L-1 2,4-D + 5 mg L-1 BA + 5 mg L-1 NAA produced a 100% of callus induction from root apices for all garlic cultivars tested....

Author(s): E. I. Metwally, M. E.  El-Denary, Y. H.  Dewir and Y. Naidoo

September 2014

In vitro cultivation technology and nutritional status of milky mushroom (Calocybe indica)

The present study was conducted to evaluate the in vivo cultivation technology, proximate composition, mineral content and spectrum analysis of edible milky mushroom of Calocybe indica. Moisture, crude protein, carbohydrate, dietary fibre, total lipids, ash, ether extract, pH, nitrogen and carbon content in mushrooms were analysed. The results were found to be in 89, 14.9, 5.36, 8.02, 4.6, 7.05, 3.15, 5.4, 3.57 and...

Author(s): Chelladurai G., Mohan Raj J. and Sasi rakha Mani M.

September 2014

Evaluation of the quality attributes of wheat composite (wheat-cassava, wheat-plantain and wheat-rice) flours in bread making

Composite flour was produced with wheat and other crops like rice, plantain and cassava at 20% substitution. The flour mixes were evaluated for proximate, physico-chemical properties and sensory evaluation was carried out on bread samples produced from these mixes. The moisture contents of these flours ranged from 11.00 to 12.30%. Also, ash and crude fibre contents ranged from 1.03 to 1.50% and 0.70 to 1.17%,...

Author(s): Ogunlakin, G. O., Abioye, V. F. and Olewepo, M. O.

September 2014

Optimization of extraction process for ustiloxins A and B from rice false smut balls using an orthogonal array design

Optimization of four factors, namely methanol concentration (A), extraction pH value (B), material-to-solvent ratio (C) and extraction times (D), for extraction of ustiloxins A and B from rice false smut balls was achieved by using an L18 orthogonal array design with three levels and four factors. The results show that the optimum conditions for ustiloxins A and B extraction should be A1B2C2D3 corresponding to methanol...

Author(s): Tijiang Shan, Xiaohan Wang, Xiaoxiang Fu, Jiajia Meng, Weibo Sun, Ali Wang, Yang Liu and Ligang Zhou

September 2014

Cytotoxicity of alkaloid fraction from Sphaeranthus amaranthoides in A549 cell line

Sphaeranthus amaranthoides (sivakaranthai) is a weed in paddy fields found in South Asia. The plant is widely exploited for its pharmacological activity. Previous research showed that the crude extract of this plant possess the antioxidant, anti-inflammatory and antibacterial activity. The current study concentrates on the cytotoxic properties of the alkaloid fraction isolated from the S. amaranthoides. The cytotoxicity...

Author(s): Y. Swarna Latha and Kommineni Nagalakshmi

September 2014

Simple and enhanced production of lignocellulosic ethanol by diluted acid hydrolysis process of pineapple peel (Ananas comosus) waste

Ethanol can be produced from a fermentation process using raw materials obtained from highly economically important plants such as corn, cassava and sugarcane, and used as an alternative energy source. These economical plants are being used less because their initial cost is still increasing. However, lignocellulosic ethanol production can alternatively be done from agricultural wastes such as corn stover, sugarcane...

Author(s): Chatchanun Niwaswong, Patiwat Chaiyamate, Peerapong Chotikosaikanon and Chalerm Ruangviriyachai,

September 2014

Toxicological aspects of geophagia in pregnancy: Putative molecular mechanism involving oxidative stress

A number of studies have demonstrated that geophagia can be beneficial and harmful. However, toxicological aspects of this earth, soil, clay eating habit are not fully investigated. In addition, low levels of antioxidants have been observed in pre-eclamptic women. Still, the molecular mechanism implicated oxidative stress in geophagia inducing pre-eclampsia has not been elucidated. Despite its absorbent properties in...

Author(s): Mossanda K. S. and Asare G. A.

September 2014

Detection of nDNA antibodies in rheumatoid arthritis patients by an immunofluorescent technique

The main objective of this study was to focus on the relationship of nDNA antibodies with rheumatoid arthritis (RA) and to determine the specificity, sensitivity, positive predictive value and negative predictive value of nDNA for the clinical diagnosis of rheumatoid arthritis. The study included a total of 40 rheumatoid arthritis cases that fulfilled the American College of Rheumatology (ACR) diagnosis criteria for...

Author(s): Hussain, Z. and Hasnain, N.