African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12294

Full Length Research Paper

Biological activity of Ipomoea pauciflora Martens and Galeotti (Convolvulaceae) extracts and fractions on larvae of Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae)

Guzmán-Pantoja Ludmila Elisa1, Guevara-Fefer Patricia3, Villarreal-Ortega María Luisa1, León-Rivera Ismael2, Aranda-Escobar Eduardo1, Martínez-Peniche Ramón Álvar4, Hernández-Velázquez Víctor Manuel1*
1Centro de investigación en Biotecnología, Universidad Autónoma del Estado de Morelos. Av. Universidad 1001, Chamilpa, 62209, Cuernavaca, Morelos, Mexico. 2Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos. Av. Universidad 1001, Chamilpa, 62209, Cuernavaca, Morelos, Mexico. 3Departamento de Biología, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, DF, Mexico. 4División de Estudios de Posgrado, Facultad de Química, Universidad Autónoma de Querétaro. Centro Universitario S/N, las Campanas, 76010, Querétaro, Querétaro, Mexico.
Email: [email protected]

  •  Accepted: 12 May 2010
  •  Published: 14 June 2010

Abstract

 Hexane, chloroform and methanol extracts of different parts of Ipomoea pauciflorawere tested for their effects on the survival and development of Fall Armyworm (Spodoptera frugiperda), a Lepidoptera pest. For seven days, neonatal larvae (grown at 27 ± 2°C with a 16: 8 (L: D) h photoperiod) were exposed to different concentrations of crude I. pauciflora extracts (ranging from 0 to 4 mg/ml) that were incorporated into an artificial diet. Surviving larvae were weighed at days 6, 9 and 13 and were maintained until moths emerged. Eleven of the 18 crude extracts showed more than 30% larval mortality. The highest mortality was produced by hexane and chloroform extracts of seeds at 4 mg/ml (96.9 and 93.8%, respectively), with LC50values of 1.85 mg/ml and 0.54 mg/ml, respectively. Fractions of both seed extracts were isolated by gravity column chromatography over silica gel and analyzed for their active compounds. Eight fractions of the hexane extract and six fractions of the chloroform extract from I. pauciflora seeds, exhibited larvicidal effects at 1 mg/ml (mortality from 33.3 to 88.9% and from 47.2 to 77%, respectively). Changes in larval weight were observed as compared with the control group. Phytochemical analysis through GC-MS and HNMR revealed the presence of fatty acids and aldehydes in the active fractions. These results indicate that the bioactive extracts from the seed of I. pauciflora can induce lethal toxicity in S. frugiperda larvae or affect the weight of the surviving larvae. 

 

Key words: Ipomoea pauciflora, bioassay, mortality, LC50 values, extracts, fractions, Spodoptera frugiperda larvae.