African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12213

Table of Content: 14 June 2010; 9(24)

June 2010

Recent progress on technologies and applications of transgenic poultry

  Manipulation of the poultry genome has the potential to improve poultry production and offer a powerful bioreactor for the production of pharmaceutical and industrial proteins in eggs. However, before realizing this, the methods for producing transgenic poultry must become routine. The direct modification of an embryo with DNA or viral vectors is possible, but this approach does not have the ability...

Author(s): Jin-Jun Li and Li-Zhi Lu

June 2010

Nano-particles - A recent approach to insect pest control

 Nanotechnology, a promising field of research opens up in the present decade a wide array of opportunities in the present decade and is expected to give major impulses to technical innovations in a variety of industrial sectors in the future. The potential uses and benefits of nanotechnology are enormous. These include agricultural productivity enhancement involving nanoporous zeolites for slow release and...

Author(s): Atanu Bhattacharyya, Asim Bhaumik, Pathipati Usha Rani, Suvra Mandaland Timothy T. Epidi

June 2010

Current trends in chloroplast genome research

  Chloroplast is an important cellular organelle of autotrophs which has an independent, circular, double-stranded DNA molecule termed as chloroplast genome. The chloroplast DNA (cpDNA) contains essential genes for its maintenance and operation. Several components of the photosystems and proteins involved in biosynthetic pathways are also encoded by the chloroplast genome. Exploring the genetic repository of...

Author(s): Asifullah Khan, Ishtiaq A. Khan, Huma Asif and M. Kamran Azim

June 2010

Identification of blast resistance expression in rice genotypes using molecular markers (RAPD & SCAR)

  Rice is the second most important cereal crop of developing countries and the staple food of about 65% of the world’s population. In this endeavor, it is important to identify resistant gene(s) with the help of markers. Once a gene is tagged with a molecular marker, it can be transferred selectively into different genetic backgrounds by marker assisted selection. For this purpose, 48 elite Indian and...

Author(s): A. Kumar, S. Kumar, R. Kumar, V. Kumar, L. Prasad, N. Kumar and D. Singh

June 2010

Isolation, identification and PCR amplification of merA gene from highly mercury polluted Yamuna river

 Mercury resistant Escherichia coli strains have been isolated from different mercury polluted sites of India and their minimum inhibitory concentration (MIC) levels were determined for HgCl2. Biochemical identification of E. coli strains was also performed with HiMedia’s biochemical identification kit for various biochemical assays, like, Citrate utilization, Lysine decarboxylase,...

Author(s): Md. Zeyaullah, Badrul Islam and Arif Ali

June 2010

Expression of infectious bovine rhinotracheitis virus glycoprotein D in bacterial cell

         Bovine Herpesvirus 1 (BHV-1) belongs to the genus of Varicellovirus and the family of Herpesviridae which contains three main gB, gC and gD genes. In order to cloning of the coding region of gD gene of IBR virus , PCR product of the open reading frame of the gene from IBR virus isolated in Iran was amplified by PCR. A 1047bp PCR product of the gD gene with EcoRI,...

Author(s): Hassan Momtaz and Mohammadreza Nazem

June 2010

Effect of solarization and vesicular arbuscular mychorrizal on weed density and yield of lettuce (Lactuca sativa L.) in autumn season

  This study was conducted on trial field of Faculty of Agriculture, Dicle University, Diyarbakir, Turkey in 2007 growing season. The experiment established on a loamy- clay soil with the seedlings of Yedikule lettuce (Lactuca sativa L.) infected by Vesicular Arbuscular Mychorrizal (VAM) fungus, Glomus intraradices. The goal was to increase the yield and quality in lettuce production. The VAM...

Author(s): Ismail Cimen, Berna Turgay and Vedat Pirinç

June 2010

Palyonological studies of the semi-desert plant species from Pakistan

 The detailed palynological description of 40 angiospermic plant species, belonging to 22 families and 38 genera were made. Out of the 22 families, 3 families were monocotyledonous and 19 dicotyledonous. The Brassicaceae and Papilionaceae were the largest families regarding number of species having four species each. In all the species except Poaceae, the pollen types were tricolporate. In case ofCalendula...

Author(s): Khalid Ahmad, Mir Ajab Khan and Nighat Shaheen

June 2010

In vitro antioxidant analysis of Achillea tenuifolia

  Achillea tenuifolia (AT) is one of the most herbs are being used by people as a traditional medicinal remedy. Antioxidant activity of AT different extracts and total flavonoid and phenol levels in the extracts were investigated in this study. Plant extracts were prepared by maceration method using ethyl acetate, methanol and methanol-water (1:1). Folin Ciocalteu reagent in terms of gallic acid equivalent...

Author(s): H. Asgarirad, F. Pourmorad, S. J. Hosseinimehr, S. Saeidnia, M. A. Ebrahimzadeh and F. Lotfi

June 2010

Characterization of Burkholderia cepacia genomovar I as a potential biocontrol agent of Ganoderma boninense in oil palm

 Burkholderia is an important bacterial genus containing several species of ecological, biotechnological and pathological interest. Bacterial isolate can be gotten from soil, water, plants and even clinics. With their taxonomy undergoing constant revision and the phenotypic similarity of several species, correct identification ofBurkholderia is difficult. Burkholderia cepacia complex (Bcc) consists...

Author(s): B. F. Azadeh, M. Sariah and M. Y. Wong

June 2010

Detection of bacterial blight resistant gene xa5 using linked marker approaches

  Rice is the primary source of food for 57% of the world’s population. Genetic resistance is important to control many kinds of pathogenic diseases. Bacterial blight caused by Xanthomonas oryzae pv oryzae (Xoo) decreases rice production by 20 – 30% and up to about 90% loss of grain weight. xa5 is an important recessive bacterial blight resistant gene, which is effective and...

Author(s): Shahzad Amir Naveed, Muhammad Babar, Ajuman Arif, Yusaf Zafar, Muhammad Sabar, Iftikhar Ali, Muhammad Chragh and Muhammad Arif

June 2010

Effects of moisture content on some physical properties of red pepper (Capsicum annuum L.) seed

 The physical properties of red pepper seed were evaluated as a function of moisture content. The average length, width and thickness were 4.46, 3.66 and 0.79 mm, respectively, at 7.27% d.b. moisture content. In the moisture range of 7.27 to 20.69% dry basis (d.b.), studies on rewetted red pepper seed showed that the thousand seed mass increased from 7.97 to 8.89 g, the projected area from 8.40 to 9.09 mm2, the...

Author(s): Necmiye Üçer, Ahmet Kılıçkan and İbrahim Yalçın

June 2010

Evaluation of phosphorus fertilizer rates for maize and sources for cowpea on different soil types in southwestern Nigeria

  Flexible phosphorus (P) fertilizer rate recommendation could be based on variations in soil characteristics that affect yield responses. Experiments were conducted in the Department of Agronomy, LAUTECH, on the effects of P rates on maize and P sources on cowpea in four soil types. On average, soil types and P rates influenced maize height and grain yield. Iwo and Egbeda soils supported taller plants than...

Author(s): G. O. Kolawole, A. O. Olayiwola, O. Ige, G. O. Oyediran and B. A. Lawal

June 2010

The effects of calcium on the expression of genes involved in ethylene biosynthesis and signal perception during tomato flower abscission

  Calcium regulation of the genes involved in ethylene biosynthesis and ethylene receptors in flower abscission zones (AZ) of wild-type tomato (Lycopersicon esculentum Mill.) was investigated in this study. Calcium treatment delayed abscission of pedicel explants. However, verapamil (VP, calcium inhibitor) treatments accelerated abscission. Results showed an increase in AZ ethylene production...

Author(s): Tao Xu, Mingfang Qi, Shuangshuang Lv, Xianhong Zuo, Jinhong Li and Tianlai Li

June 2010

Measuring the marketing performances of state forest enterprises in Turkey

 Today, any enterprise has to consider marketing as important as production. Production and marketing are two complementary activities in enterprises. The increase in marketing effectiveness is achieved through measuring the activity outcomes that can be described as marketing performance. Because marketing is a social science and, therefore, determining the marketing performance system concretely, examining it and...

Author(s): Devlet Toksoy and Hasan Ayyildiz

June 2010

Soil loss prediction using universal soil loss equation (USLE) simulation model in a mountainous area in Ağlasun district, Turkey

  Land degradation and soil loss is a global event. Human induced pressures on the natural ecosystems are still in progress as well as conservation efforts. The need for sufficient knowledge and data for decision makers is obvious hence the present study was carried out. The study area, the Ağlasun district, is in the middle west of Turkey and is characterized by a cold and sub-humid Mediterranean climate. The...

Author(s): Sezgin Hacısalihoğlu, Ahmet Mert, M. Güvenç Negiz and Bart Muys

June 2010

The color changes on varnish layers after accelerated aging through the hot and cold-check test

 The purpose of this paper is to determine the color changes of cellulosic, polyurethane and acrylic varnish layers which were applied on the surfaces of Scots pine (Pinus sylvestris L.), Eastern beech (Fagus orientalis L.) and sessile oak (Quercus petraea L.) wood material after accelerated aging effects through the hot and cold-check test. For this purpose, test samples were firstly kept at 50...

Author(s): Mehmet Budakçı, Derya Sevim Korkut  and Raşit Esen

June 2010

Cold storage of rooted and non-rooted carnation cuttings

  The specific objectives of this study were to evaluate the effect of storage duration on the survival rate of rooted cuttings and to determine the rooting and survival rates of non-rooted cuttings for two standard carnation cultivars (that is., Dianora and Vittorio). The survival rates of rooted cuttings showed differences depending on the cultivar tested. Specifically, the Vittorio cultivar had a better...

Author(s): Murat Zencirkiran

June 2010

Comparative study of Aspergillus niger and Penicillium sp. in the biodegradation of automotive gas oil (AGO) and premium motor spirit (PMS)

  The comparative study of Aspergillus niger and Penicillium sp. in the biodegradation of automotive gas oil (AGO) and premium motor spirit (PMS) was carried out to ascertain the effectiveness of using these microorganisms in cleaning and restoring the ecosystem when polluted by petroleum products. These fungi were observed to grow at all concentrations of the petroleum products (pollutant)...

Author(s): Isitua, C. C. and Ibeh, I. N.

June 2010

Isolation of two Kocuria species capable of growing on various polycyclic aromatic hydrocarbons

  Different samples collected from crude oil contaminated beach were enriched for isolation of bacterial strains capable of growing on naphthalene, phenanthrene and fluoranthene. Respiratory reduction of WST-1{4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate} to a colored formazan showed that one isolated strain CMG2028, identified as Kocuria flava by 16s...

Author(s): Rifat Zubair Ahmed, Nuzhat Ahmed and Geoffrey Michael Gadd

June 2010

Isolation of rapid growing mycobacteria from soil and water in Iran

 A total of 350 soil samples were collected from different part of Uremia city and suburbs. We used 3% sodium lauryl sulfate and 1% NaOH for decontamination of soil samples. Of 350 samples, mycobacteria were isolated from 65 (18.3%) specimens. Mycobacterium fortuitum with 18(5.14) strains yielded the highest frequency of isolation. The other isolates were: Mycobacterium...

Author(s): Mohammad Rahbar, Aboulfath Lamei, Homayoon Babazadeh and Shoherh Afshar Yavari

June 2010

Macrofungal diversity of Bolu Abant Nature Park (Turkey)

  This study was based on materials of macrofungi collected from Bolu Abant Nature Park between 2008 and 2009. As a result of field and laboratory studies, 103 taxa belonging to 34 families were identified. Five (5) taxa belong to Ascomycota and 98 to Basidiomycota.    Key words:  Biodiversity, macrofungi, taxonomy, Bolu Abant Nature Park.

Author(s): Hüseyin Servi, Ilgaz Akata and Barbaros Çetin

June 2010

Analysis of food components of freeze-dried Alaska Pollack (Hwangtae)

 Physiochemical properties of freeze-dried Alaska Pollack (Hwangtae) were investigated. The moisture, crude ash and crude fat content of Hwangtae were 12.53, 5.91 and 0.94%, respectively. Contents of saccharides were found to be 3.69 mg/100 g in dried weight of glucose and 5.71 mg/100 g in dried weight of sucrose. Minerals in 100 g dry weight of Hwangtae include phosphorus (806.5 mg), calcium (612.7 mg), potassium...

Author(s): Ju-Sung Kim, Kyoung Su Kim and Myong-Jo Kim,

June 2010

Enhanced regeneration in explants of tomato (Lycopersicon esculentum L.) with the treatment of coconut water

  A standardized protocol was developed to enhance the in vitro regeneration efficiency of five varieties of tomato from hypocotyls and leaf disc with the involvement of coconut water. Different concentrations of IAA and kinetin were used alone and in combination with 12% of coconut water. Significant differences for regeneration, time taken to regenerate and number of leaf primordial were observed...

Author(s): Amber Afroz, Zubeda Chaudhry, Umer Rashid, Muhammad Rashid Khanand Ghulam Muhammad Ali

June 2010

Antiglycation Activity of Otostegia persica (Burm.) Boiss

 Diabetes mellitus is a common endocrine disorder characterized by hyperglycemia and long-term complications affecting the eyes, nerves, blood vessels, skin and kidneys. Increased glycation of proteins and accumulation of advanced glycation endproducts (AGEPs) have been implicated in the pathogenesis of diabeticcomplications. Glycation and AGEP formation are also accompanied by the...

Author(s): Seyed Abdul Majid Ayatollahi, , Farzad Kobarfard, , Jinous Asgarpanahand Muhammad Iqbal Choudhary

June 2010

Preliminary study and Identification of insects’ species of forensic importance in Urmia, Iran

  The proper identification of the insect and arthropod species of forensic importance is the most crucial element in the field of forensic entomology. The main objective in this study was the identification of insects’ species of forensic importance in Urmia (37°, 33′ N. and 45°, 4′, 45″ E.) and establishment of a preliminary data-base for forensic entomology purposes in...

Author(s): A. Tüzün, F. Dabiri and S. Yüksel

June 2010

Biological activity of Ipomoea pauciflora Martens and Galeotti (Convolvulaceae) extracts and fractions on larvae of Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae)

 Hexane, chloroform and methanol extracts of different parts of Ipomoea pauciflorawere tested for their effects on the survival and development of Fall Armyworm (Spodoptera frugiperda), a Lepidoptera pest. For seven days, neonatal larvae (grown at 27 ± 2°C with a 16: 8 (L: D) h photoperiod) were exposed to different concentrations of crude I. pauciflora extracts (ranging from 0 to 4 mg/ml)...

Author(s): Guzmán-Pantoja Ludmila Elisa, Guevara-Fefer Patricia, Villarreal-Ortega María Luisa, León-Rivera Ismael, Aranda-Escobar Eduardo, Martínez-Peniche Ramón Álvar, Hernández-Velázquez Víctor Manuel

June 2010

An overview of medical image processing methods

  Since human life is worthier than all things, efforts on virtual animation and visualization of human body’s viscera, without surgical interference to diagnose a disease is very important. Recently, modern medical instruments are able to produce views which can be used for better diagnoses and accurate treatment. Various standards were formed regarding these instruments and end products that are being...

Author(s): Hakan Hadi Maraş, Yüksel Maraş, Erdem Emin Maraş, Bahadır Aktuğ, Süleyman Sırrı Maraş and Ferruh Yildiz

June 2010

Comparative study on growth and survival of larval and juvenile Dicentrarchus labrax rearing on rotifer and Artemia enriched with four different microalgae species

 In the present study, two experiments were carried out, the first one at age from 4th to 24th days post hatching (dph) which include Dicentrarchus labrax larvae rearing on rotifer and Artemia enriched with four types of algae as follows:Chlorella salina, Dunaleilla salina, Nannochloropsis salina and Tetraselmis chuii(ch1, D1, N1 and T1). At the end of the...

Author(s): M. I. Zaki and H. Saad