Abstract

Invertase (β-fructofuranosidase) finds major applications in food, cosmetics, and pharmaceutical industries. In the present study, fermentation conditions for invertase production from Saccharomyces cerevisiae Angel using date syrup in shake flask were investigated. The effect of time period (0-96 h), carbon sources (sucrose, date syrup, maltose, galactose, and glucose), nitrogen sources including yeast extract, peptone, urea, meat extract, and ammonium sulphate, pH (4 to 6.5), temperature (30 to 40°C), date syrup concentration (5 to 50 g/L), and inoculum size (1 to 10% v/v) were optimized. Optimum conditions for yeast growth and invertase yield were 20 g/L date syrup, 10 g/L yeast extract, temperature of 37°C, pH 5.5, and 60 h fermentation time. In addition, effect of date syrup concentration and size of inoculum were also optimized. Highest invertase titer of ~190 U/mL were obtained when 40 g/L of date syrup and 10% v/v inoculum were used. These results show great practical potential for invertase production by saving enzyme production cost.

Key words: Saccharomyces cerevisiae Angel, invertase, date syrup, β-fructofuranosidase.