Full Length Research Paper
Abstract
Glucose metabolism plays a crucial role in the process of avermectin B1abiosynthesis. Controlling glucose feeding based on oxygen uptake rate (OUR) was established to improve the efficiency of avermectin B1a production. The result showed that avermectin B1a production was greatly enhanced by OUR control strategy. In the glucose feeding phase, OUR was maintained at approximate 12 mmol/L/h, which was conducive to avermectin B1a biosynthesis. Using this OUR control strategy, an adequate supply of organic acid precursors produced avermectin B1a 5228 U/mL, which was 22.8% higher than that of the control (batch fermentation, 4256 U/mL) on industrial scale.
Key words: Avermectin B1a, glucose feeding, oxygen uptake rate, industrial scale.
Abbreviation
OUR, Oxygen uptake rate; CER, carbon dioxide evolution rate; RQ,respiratory quotient; DO, dissolved oxygen; OUR-AA, oxygen uptake rate-amino acids; PMV, packed mycelium volume; HPLC, high performance liquid chromatography; MDW, mycelial dry weight; PKS, polyketide synthetase.
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