African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Genetic control of protein, oil and fatty acids content under partial drought stress and late sowing conditions in sunflower (Helianthus annuus)

P. Haddadi1,2,3, B. Yazdi-samadi2, N. B. Langlade1, M. R. Naghavi2, M. Berger4, A. Kalantari2, A. Calmon4, P. Maury5, P. Vincourt1 and A. Sarrafi3*
1Laboratoire des Interactions Plantes Micro-organismes, UMR CNRS-INRA 2594/441, Chemin de Borde-Rouge -Auzeville -BP 52627- 31326 Castanet Tolosan, France. 2Agronomy and Plant Breeding Department, Faculty of Agriculture, University of Tehran, Karaj, Iran. 3INP-ENSAT, IFR 40, Laboratoire de Symbiose et Pathologie des Plantes (SP2), 18Chemin de Borde-Rouge, F-31326 Castanet Tolosan, France. 4UMR 1054 INRA/EIP - Laboratoire d’Agrophysiologie, Ecole d’Ingénieurs de Purpan. 75, Voie du TOEC, BP 57611- 31076 Toulouse Cedex 3, France. 5INP-ENSAT, INRA, UMR 1248, Agrosystèmes et Développement Territorial, 31326 Castanet Tolosan, France.
Email: [email protected]

  •  Accepted: 06 July 2010
  •  Published: 04 October 2010

Abstract

The purpose of the present study was to map quantitative trait locus (QTLs) associated with percentage of seed protein, oil and fatty acids content under different conditions in a population of recombinant inbred lines (RILs) of sunflower. Three independent field experiments were conducted with well-, partial-irrigated and late-sowing conditions in randomized complete block design with three replications. High significant variation among genotypes is observed for the studied traits in all conditions. Several specific and non-specific QTLs for the aforementioned traits were detected. Under late-sowing condition, a specific QTL of palmitic acid content on linkage group 6 (PAC-LS.6) is located between ORS1233 and SSL66_1 markers. Common chromosomic regions are observed for percentage of seed oiland stearic acid content on linkage group 10 (PSO-PI.10 and SAC-WI.10) and 15 (PSO-PI.15 and SAC-LS.15). Overlapping occurs for QTLs of oleic and linoleic acids content on linkage groups 10, 11 and 16. Seven QTLs associated withpalmitic, stearic, oleic and linoleic acids content are identified on linkage group 14. These common QTLs are linked to HPPD homologue, HuCL04260C001.Coincidence of the position for some detected QTLs and candidate genes involved in enzymatic and non-enzymatic antioxidants would be useful for the function of the respective genes in fatty acid stability.

 

Key words: Sunflower, quantitative trait locus, simple sequence repeats, oil content, protein content, fatty acids.

Abbreviation

QTL, Quantitative trait locus; CIM, composite interval mapping;RIL,  recombinant inbred line; MRILs, mean of RILs; MP, mean of their parents;NIRS, near-infrared reflectance spectrometry; HPPD, p-hydroxyphenylpyruvate dioxygenase; EST, expressed sequence tags; HPP, p-hydroxyphenylpyruvate;HGA, homogentisic acid SSR, simple sequence repeats; PSP, percentage of seed protein; PSO, percentage of seed oil; PAC, palmitic acid content; SAC, stearic acid content;  OAC, oleic acid content; LAC, linoleic acid content; RFLP, restriction fragment length polymorphism; AFLP, amplified fragment length polymorphisms.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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