Abstract

Targeted screening of EGFR compounds has become one of the medical research focuses for tumor therapy. A431, which naturally expresses high levels of EGFR, was compared with the stably high expressing EGFR cell line HEK293. Flow cytometry was used to analyze cell growth and Western blot was used to determine expression of EGFR, p-EGFR and p-ERK. Cellular membrane chromatography analysis revealed longer on-peak chromatographic and greater on-peak area of A431. Results demonstrated that the high-expression EGFR cell line A431 improved sensitivity of cellular membrane chromatography when screening drugs targeted to EGFR.

Key words: High-EGFR expression, drug-targeted screening, A431 cell line and Taspine.

Abbreviation

EGFR, Epidermal growth factor receptor; EPR, epiregulin; MAPK,mitogen-activated protein kinase; PKC, protein kinase C; PLC-γ, phospholipase C gamma; STAT, Signal transducer and activator of transcription; NSCLC, non-small cell lung cancer; EDTA, ethylene diamine tetraacetic acids; RT-PCR, reverse transcriptase- polymerase chain reaction; FBS, fetal bovine serum; ECL, enhanced chemiluminescence; CMSP, cell membrane stationary phase; PBS, phosphate buffer saline; HEK293, human embryonic kidney 293 cells.