African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12392

Full Length Research Paper

Characterization and identification of Russula firmula and Russula postiana from Himalayan moist temperate forests of Kashmir

  Zahoor Ahmad Itoo1, 2*, Zafar A Reshi1 and Khurshid Iqbal Andrabi2        
  1Biological Invasions Research Lab, Department of Botany, University of Kashmir, Hazratbal, Srinagar-190006, J&K, India. 2Department of Biotechnology University of Kashmir, Hazratbal, Srinagar-190006, J&K, India.
Email: [email protected], [email protected]

  •  Published: 05 June 2013

Abstract

 

Two ectomycorrhizal species of genus RussulaRussula postiana and Russula firmula (Basidiomycota, Agaricales) have been characterized and identified from Kashmir Himalaya using morpho-anatomical and molecular methods targeting its rDNA. The target internal transcribe spacer (ITS)-rDNA of both species was amplified using polymerase chain reaction (PCR) with universal fungal primers (ITS1 and ITS4), which generated 700 bp fragments. After sequencing of amplified product, the initial blast analysis revealed and confirmed the identification of both species by comparing the sequences of these respective species present in GenBank. Further, in phylogenetic analysis both species distinctly clustered with their respective groups. Morphological characteristics like shape, size and colour of pileus, stipe and gills, basidiospore size of both the species was measured and compared with data given in literature.

 

Key words: Ectomycorrhizal, morpho-anatomical, sequencing, phylogenetic.