African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12340

Table of Content: 5 June 2013; 12(23)

June 2013

Photodynamic therapy: A new light for the developing world

  In one article, photodynamic therapy (PDT) was commended as the most suitable method for cancer therapy in the Developing World. PDT is cost effective and simple to use. Unlike chemotherapy, no special training is required for nurses, and no post treatment course in intensive care. No engineer, computerized dosimetry computations, or additional costs for isotope re-treatment are required, as in radiotherapy....

Author(s):   Songca, S. P. and Oluwafemi, O. S.  

June 2013

Engineering insect-resistant crops: A review

  Insect pests cause significant damage to crops world-wide. This is despite integrated pest management strategies combining such control measures as chemical control, use of resistant varieties and other measures. Other control measures such as use of genetically modified crops are being adopted. Transgenic crops engineered for enhanced levels of resistance to insect pests have the potential to offer large...

Author(s):   Derick G. M. George  

June 2013

Practical problems and their solutions in studying the biology of the mealybug Paracoccus burnerae (Brain) (Hemiptera: Pseudococcidae)

  Mealybugs are among the most widespread and important pests of plants in commercial glasshouses and conservatories. They are also important quarantine pests that hinder international trade of fruits, vegetables and ornamental plants.Studies on their biology are important because they attack a wide range of plants and are generally difficult to control. Some of the many problems generally encountered in...

Author(s):   T. Johnson and J. H. Giliomee    

June 2013

Interleukin (IL)-17A and IL-17F and asthma in Saudi Arabia: mRNA transcript levels and gene polymorphisms

  Asthma is a multifactorial disorder and both genetic and environmental factors contribute to its development. The present study explored cytokines interleukin (IL)-17A and IL17F levels as usable parameters for the diagnosis of asthmatics Saudi patients. Blood samples were collected from 100 asthma patients and 100 matched controls. The transcript mRNA levels in whole blood were determined by real-time...

Author(s):   MD Bazzi, MA Sultan, N Al Tassan, M Alanazi, A Al-Amri, MS Al-Hajjaj, S Al-muhsen, K Alba-Concepcion and A Warsy  

June 2013

Assessment of genetic diversity in Isabgol (Plantago ovata Forsk.) using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers for developing crop improvement strategies

  Isabgol (Plantago ovata F., family: Plantaginaceae) is one of the most importantmedicinal plants of South Asia. Two DNA based molecular marker techniques, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), were used to study the genetic diversity among genotypes of Isabgol. A total of 38 polymorphic primers (22 random and 16 ISSR) were used. Amplification...

Author(s):   Vineet Kaswan, Arunabh Joshi and S. R. Maloo  

June 2013

Discovery AP2/ERF family genes in silico in Medicago truncatula

  Medicago truncatula is a legume model plant due to its small genome, and it has been used to study the molecular events of legume biology. As a crucial plant-specific gene family, AP2/EREBP transcription factors (TFs) are important for plant development and biotic and abiotic stress responses. The purpose of the work was to determine AP2/ERF family genes in silico of M. truncatula, and...

Author(s):   Zhifei Zhang, Qian Zhou, Zhijian Yang and Jingpeng Jiang  

June 2013

Characterization and identification of Russula firmula and Russula postiana from Himalayan moist temperate forests of Kashmir

  The biochemical changes influencing shoot regeneration in mangroves, viz. Bruguiera parviflora (Roxb) Wt. & Arn. Ex Griff. , Kandelia candel (L.) Druce. and Rhizophora apiculata Bl. was studied by  analysing antioxidant enzyme activities during induction of in vivo multiple shoot regeneration. The impact of NaCl and proline on enzyme behavior was...

Author(s):   Uday Chand Basak, Pramodini Rout and Ajay Kumar Mahapatra  

June 2013

Characterization and identification of Russula firmula and Russula postiana from Himalayan moist temperate forests of Kashmir

  Two ectomycorrhizal species of genus Russula: Russula postiana and Russula firmula (Basidiomycota, Agaricales) have been characterized and identified from Kashmir Himalaya using morpho-anatomical and molecular methods targeting its rDNA. The target internal transcribe spacer (ITS)-rDNA of both species was amplified using polymerase chain reaction (PCR) with universal fungal...

Author(s):   Zahoor Ahmad Itoo, , Zafar A Reshi and Khurshid Iqbal Andrabi        

June 2013

Response of barley plants to foliar application of growth regulators mixture of indole acetic acid, naphthalene acetic acid and zinc

  This study was conducted to show the effect of foliar application with a growth mixture containing indole acetic acid (IAA) and naphthalene acetic acid (NAA) at the levels of 0 + 0, 50 + 25, 100 + 50 and 150 + 75 ppm, respectively, and/or zinc concentration of 0, 50, 100, and 150 ppm sprayed three times after 20, 40 and 60 days of sowing, on growth, yield as well as some physiological and...

Author(s):   Mona, E. Eleiwa, Maymona, A. Kord  and S. A. Ibrahim  

June 2013

Calcium carbide (CaC2): Effect on fruit set and yield of mango (Mangifera indica L.) cv. Langra

  Application of slow release calcium carbide (CaC2) to the soil are thought to improve plant growth and yield through the generation of acetylene, which inhibits nitrification, thereby reducing nitrogen losses which may be converted to physiologically significant concentrations of the plant growth regulator ethylene. The effects of different application rates of slow release of CaC2 on the growth and...

Author(s):   Waqas Akram, Saeed Ahmad, Muhammad Yaseen, Waqas Ahmad, Waqar Ahmad, C.M.Ayub and Muhammad Asif Shehzad  

June 2013

Gene expression associated with the biosynthetic pathway of abscisic acid, gibberellin and ethylene during lettuce seed development

  Seed development may be divided up into embryonic morphogenesis and seed maturation, while diverse genes were connected and disconnected in order to bring about such processes.  Features such as desiccation tolerance, dormancy and germination capacity are acquired throughout development and regulated by the presence and interaction of phytohormones such as abscisic acid (ABA), gibberellin (GA) and...

Author(s):   Aline da Consolação Sampaio Clemente, Renato Mendes Guimarães, Denize Carvalho Martins, André Almeida Lima, Luiz Antonio Augusto Gomes and Alexana Baldoni    

June 2013

A practical evaluation of detergent and disinfectant solutions on cargo container surfaces for bacteria inactivation efficacy and effect on material corrosion

  Cleaning and disinfection agents were evaluated against selected bacteria on three surfaces: aluminium, stainless steel and fibre re-enforced plastic, used as cargo container linings and to access their effect on the surface integrity. Nine sanitation chemical solutions: benzalkonium chloride, sodium hypochlorite, nitric acid, levulinic acid, peracetic acid sodium hydroxide, sodium dodecyl sulphate, AT...

Author(s):   Stephen Abban, Mogens Jakobsen, and Lene Jespersen  

June 2013

Bioinsecticide activity of Bacillus thuringiensis isolates on tomato borer, Tuta absoluta (Meyrick) and their molecular identification

  Twelve bacterial isolates were isolated from dead larvae of Tuta absoluta (4th instar) from tomato cultivated fields at Fayoum Governorate, Egypt. All isolates were preliminarily identified as members of the genus Bacillus based on morphological and biochemical characteristics. According to the results of the pathogenicity of Bacillusisolates against different larval instars...

Author(s):   Narmen A. Youssef and G. M. Hassan  

June 2013

Quality protein maize (QPM) seeds grown in Côte d’Ivoire: A source of high value edible oil

  The search for new sources of oil with improved properties has focused our attention on the characteristics of oils extracted from white and yellow quality protein maize (QPM) seeds, two hybrids of classic maize (Zea mays L.). Physicochemical parameters of extracted oils were respectively as follow: refractive index (1.47 ± 0.00), free fatty acids (FFA) (1.4 ± 0.00 %),...

Author(s):   Mariame Cissé, Lessoy T. Zoué, Rose-Monde Megnanou, René Y. Soro and Sébastien L. Niamké  

June 2013

Use of natural fluorescent triacyglycerols from Parinari glaberrimum to detect low lipase activity in Brassica napus seedlings

  Lipases (triacylglycerol acyl hydrolass EC 3.1.1.3) are defined as enzymes that catalyse the hydrolysis of triacylglycerols, releasing long-chain fatty acids. Germinating oilseeds have been explored as a possible source of lipases for biotechnological processing of oils and fats. However, purification and sensitive assays to detect a true lipase activity in plant cellular homogenates and culture media are...

Author(s):   Belguith, H., Jridi, T., Frédéric, B. and Ben Hamida J.  

June 2013

Applying machine learning to predict patient-specific current CD4 cell count in order to determine the progression of human immunodeficiency virus (HIV) infection

  This work shows the application of machine learning to predict current CD4 cell count of an HIV-positive patient using genome sequences, viral load and time. A regression model predicting actual CD4 cell counts and a classification model predicting if a patient’s CD4 cell count is less than 200 was built using a support vector machine and neural network. The most accurate regression and...

Author(s):   Yashik Singh, Nitesh Narsai and Maurice Mars  

June 2013

Haematological evaluation of Wistar rats exposed to chronic doses of cadmium, mercury and combined cadmium and mercury

  Cadmium and mercury present in the environment, cause blood disorders. This study was conducted to evaluate the influence of cadmium, mercury and their combination on hematological parameters of Wistar rats. For this purpose, two different doses of each metal and their combination were administered orally for 28 days to six groups of five rats each. Two groups (A and B) were respectively exposed to...

Author(s):   Hounkpatin, A. S. Y., Edorh, P. A., Guédénon, P., Alimba, C. G., Ogunkanmi, A., Dougnon, T. V., Boni, G., Aissi, K. A., Montcho, S., Loko, F., Ouazzani, N., Mandi, L., Boko, M. and Creppy, E. E.  

June 2013

Isolation of α-amylase from malted rice (Wita 7) extract using cassava starch column procedure

  The current study investigated the production and purification of α-amylase from malted rice (Wita 7) extract. In the course of the study, malting parameters such as germination energy, malt yield as well as malt loss of rice (Wita 7) were estimated. An appraisal of α-amylase production during malting with rice (Wita 7) revealed that, the production level peaked on the 8th day of germination...

Author(s):   E. Asante , A.A. Adjaottor  and M. Y Woode  

June 2013

Evaluation of antimicrobial activity exploration of the leaves extracts of Borreria articularis (Linn. F.)

  Publisher’s Retraction  Retraction: Evaluation of antimicrobial activity exploration of the leaves extracts of  Borreria articularis (Linn. F.) Abul Kalam Azad 1 *, Wan Azizi mohd Wan Sulaiman 1  and Tengku Muhamad Faris  Syafiq bin Tengku Zakaria 2   1 Department of Basic Medical Sciences, Faculty of Pharmacy, International...

Author(s): Abul Kalam Azad, Wan Azizi mohd Wan Sulaiman and Tengku Muhamad Faris Syafiq bin Tengku Zakaria