African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Effect of age on somatic embryogenesis from immature zygotic embryos of 5 Turkish triticale genotypes

Mehmet Atak1, Muharrem Kaya2, Khalid Mahmood Khawar3*, Sevil Saglam3, Sebahattin Özcan3 and Cemalettin Yasar Ciftci3
  1Department of Field Crops, Faculty of Agriculture, Mustafa Kemal University, 31034 Hatay, Turkey. 2Department of Field Crops, Faculty of Agriculture, Suleyman Demirel University, Isparta, Turkey. 3Department of Field Crops, Faculty of Agriculture, University of Ankara, 06110 Dışkapı- Ankara, Turkey.
Email: [email protected]

  •  Accepted: 25 March 2008
  •  Published: 03 June 2008

Abstract

 

Triticale is an important cereal crop grown throughout the world. The study reports somatic embryogenesis from immature zygotic embryos of 5 Turkish triticale genotypes. The explants were initially cultured on MS medium supplemented with 2 mg dm-3  2,4-D, 500 mg dm-3 glutamine, 100 mg dm-3casein hydrolysate, 2% sucrose and 7% agar for two weeks in the dark. The temperature was maintained at 24±2ºC. Thereafter, the developing embryogenic calli were transferred to MS medium without 2,4-D to achieve embryogenesis under light intensity of 30 000 lux in 16 h light 8 h dark photoperiod at 24±2ºC for 2 weeks. The developing somatic embryos were then transferred to MS medium to mature them and obtain plantlets. The highest number of 9.63 somatic embryos per explant were recorded for genotype LAD388 from 14 - 16 days old explants. The experiment clearly established that 14 - 16 days old explants were superior compared to 17 - 19 days old explants in terms of number of somatic embryos per explant. The matured plantlets were acclimatised in the greenhouse on organic matter rich soil mix contained in pots.

 

Key words: Triticale, immature zygotic embryos, somatic embryogenesis, age.

Abbreviation

MS medium, Murashige and Skoog medium; 2,4-D, 2, 4- dichlorophenoxyacetic acid