Abstract

RAPD profile revealed variation among the individuals of a single clone of micro propagated plants of Trichodesma indicum. Callus was developed from a single zygotic embryo differentiated into multiple micro shoots on Murashige and Skoog’s medium supplemented with 2.32 µM kinetin. A single node excised from a micro shoot produced multiple shoots after three sub cultures on MS medium containing 0.23 µM kinetin. Morphologically, all the in vitro raised plantlets appeared alike except size variation. Random amplified polymorphic DNA (RAPD) profile of a clone of 10 plantlets prepared with MAP 1 - 20 primers amplified 119 bands: 117 monomorphic and 2 polymorphic. Perhaps, variation occurred during subculture of nodal segments rather than differentiation of shoots from callus.

Key words: Trichodesma indicum, RAPD, MAP-primers, somaclonal variation, micro propagation.

Abbreviation

RAPD, Random amplified polymorphic DNA;  PCR, polymerase chain reaction; CTAB, cetyl trimethyl ammonium bromide.