Effect of banana bunchy top virus (BBTV) on morpho-physiological characteristics of banana (Musa sp.) cv., Basrai plants was assessed. Healthy and BBTV infected samples of banana were collected from its open fields and micro-propagated aseptically. These plantlets were established in wire-house for three months. Presence of BBTV was confirmed by polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Micro-propagation efficiency of BBTV-infected plants was observed in less than healthy plants under in-vitro as well as in-vivo. Significant reduction in plant height, fresh weight (F wt) and dry weight (D wt) of leaf was observed in BBTV infected plants. A co-relation of peroxidase (POX) activity was observed with total carotenoids that increased in BBTV infected plants, while chlorophyll contents decreased significantly. Nitrate reductase activity also decreased with increase in proline contents in BBTV-stresses plants (p<0.05). Meanwhile, reducing sugars also increased but not-significantly. Bunchy top virus infection in banana therefore resulted in alteration of growth related physiological traits that led to retardation of plant growth.
Key words: Ex-vitro, Musa sp., micro-propagation, photosynthetic pigments, BBTV, peroxidases, total proteins, reducing sugars, cell size.
Abbreviations: MS, Murashige and Skoog; BAP, benzyleaminopurine; PCR, polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay; BBTV, banana bunchy top virus; RWC, relative water contents; F Wt, fresh weight; D Wt, dry weight; NRA, nitrate reductase activity; POX, peroxidases; Na2CO3, sodium carbonate.
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