Certain growth related attributes of bunchy top virus infected banana under ex-vitro conditions

Ikram-ul-Haq*; Shahrukh; Um-E-Aiman; Saifullah Khan; Nazia Parveen; Madiha Fatima; Muhammad Umar Dahot

doi:10.5897/AJB11.2093

African Journal of
Biotechnology

Abbreviation: Afr. J. Biotechnol.
Language: English
ISSN: 1684-5315
DOI: 10.5897/AJB
Start Year: 2002
Published Articles: 12487

Full Length Research Paper

Certain growth related attributes of bunchy top virus infected banana under ex-vitro conditions

Ikram-ul-Haq*

Ikram-ul-Haq*
Institute of Biotechnology and Genetic Engineering (IBGE), University of Sindh, Jamshoro-76080, Pakistan.
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Shahrukh

Shahrukh
Institute of Biotechnology and Genetic Engineering (IBGE), University of Sindh, Jamshoro-76080, Pakistan.
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Um-E-Aiman

Um-E-Aiman
Institute of Biotechnology and Genetic Engineering (IBGE), University of Sindh, Jamshoro-76080, Pakistan.
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Saifullah Khan

Saifullah Khan
Plant Tissue Culture and Biotechnology Wing, H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan.
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Nazia Parveen

Nazia Parveen
Department of Statistics, University of Sindh, Jamshoro-76080, Pakistan.
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Madiha Fatima

Madiha Fatima
Institute of Biotechnology and Genetic Engineering (IBGE), University of Sindh, Jamshoro-76080, Pakistan.
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Muhammad Umar Dahot

Muhammad Umar Dahot
Institute of Biotechnology and Genetic Engineering (IBGE), University of Sindh, Jamshoro-76080, Pakistan.
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Article Number - 6B9E23F44434
Vol.13(18), pp. 1876-1882 , April 2014
https://doi.org/10.5897/AJB11.2093

Received: 07 July 2011
Accepted: 14 November 2011
Published: 30 April 2014

Abstract

Effect of banana bunchy top virus (BBTV) on morpho-physiological characteristics of banana (Musa sp.) cv., Basrai plants was assessed. Healthy and BBTV infected samples of banana were collected from its open fields and micro-propagated aseptically. These plantlets were established in wire-house for three months. Presence of BBTV was confirmed by polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Micro-propagation efficiency of BBTV-infected plants was observed in less than healthy plants under in-vitro as well as in-vivo. Significant reduction in plant height, fresh weight (F wt) and dry weight (D wt) of leaf was observed in BBTV infected plants. A co-relation of peroxidase (POX) activity was observed with total carotenoids that increased in BBTV infected plants, while chlorophyll contents decreased significantly. Nitrate reductase activity also decreased with increase in proline contents in BBTV-stresses plants (p<0.05). Meanwhile, reducing sugars also increased but not-significantly. Bunchy top virus infection in banana therefore resulted in alteration of growth related physiological traits that led to retardation of plant growth.

Key words: Ex-vitro, Musa sp., micro-propagation, photosynthetic pigments, BBTV, peroxidases, total proteins, reducing sugars, cell size.

Abbreviation

Abbreviations: MS, Murashige and Skoog; BAP, benzyleaminopurine; PCR, polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay; BBTV, banana bunchy top virus; RWC, relative water contents; F Wt, fresh weight; D Wt, dry weight; NRA, nitrate reductase activity; POX, peroxidases; Na₂CO₃, sodium carbonate.