African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Short Communication

Isolation of Mycobacterium tuberculosis complex (MTBC) from dairy cows in China

Yingyu Chen1,2, Siguo Liu3, Yujiong Wang4, Yanfen Du3, Min Li4, Shi Cheng1, Huanan Wang3, Qin Xie4, Huanchun Chen1 and Aizhen Guo1
  1The State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, P. R. of China. 2College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, P. R. of China. 3State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. of China. 4Key Laboratory of Animal Disease Control and Prevention, Ministry of Education, School of Life Science, Ningxia University, Yinchuan, P. R. of China.
Email: [email protected]

  •  Accepted: 22 June 2012
  •  Published: 08 November 2012

Abstract

 

Eleven thousand five hundred and eighty non-blood samples from dairy cows were subjected to mycobacterium culture and genotyping. As a result, a total of 142 isolates of Mycobacterium tuberculosis complex (MBTC) were identified. Among them, 65 were Mycobacterium tuberculosis, while 77 Mycobacterium bovis. The genotype ofM. tuberculosis strains was mainly Beijing family. In addition, the isolation rates of MTBC were 33.89% for lung lymph nodes, 2.81% for nasal swabs, and 3.95% for pharyngeal swabs from cattle positive to tuberculin skin test, respectively. This evidence implied that M. tuberculosis infection in cattle is a new risk to public health and should be paid more attention.

 

Key words: Mycobacterium tuberculosis complex, cows, tuberculosis, zoonosis

Abbreviation

TB, Tuberculosis; MTBC, Mycobacterium tuberculosis complex; TST, tuberculin skin test; PPD, purified protein derivative; PCR, polymerase chain reaction

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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