African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Bioepoxidation of isosafrol catalyzed by radish and turnip peroxidases

Micael Nunes Melo
  • Micael Nunes Melo
  • Universidade Tiradentes, Instituto de Tecnologia e Pesquisa. Laboratorio de Engenharia de Bioprocessos. Av. Murilo Dantas, 300, 49032-490, Farolandia, Aracaju, SE, Brazil.
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Lucas Costa Lopes
  • Lucas Costa Lopes
  • Universidade Tiradentes, Instituto de Tecnologia e Pesquisa. Laboratorio de Engenharia de Bioprocessos. Av. Murilo Dantas, 300, 49032-490, Farolandia, Aracaju, SE, Brazil.
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ClŠudio Dariva
  • ClŠudio Dariva
  • Universidade Tiradentes, Instituto de Tecnologia e Pesquisa. Laboratorio de Engenharia de Bioprocessos. Av. Murilo Dantas, 300, 49032-490, Farolandia, Aracaju, SE, Brazil.
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Jonathan dos Santos Girardi
  • Jonathan dos Santos Girardi
  • Programa de Pos-Graduacao em Engenharia Quimica, Departamento de Engenharia Quimica e Engenharia de Alimentos, Universidade Federal de Santa Catarina (UFSC), Campus Universitario, Trindade, Florianopolis, SC, 88040-900, Brazil.
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Angelica Maria Lucchese
  • Angelica Maria Lucchese
  • Laboratorio de Quimica de Produtos Naturais e Bioativos, Universidade Estadual de Feira de Santana (UEFS), Av. Transnordestina S/N, Novo Horizonte, 44036-900, Campus Universitario, Feira de Santana, BA, Brazil.
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Heiddy Marquez Alvarez
  • Heiddy Marquez Alvarez
  • Laboratorio de Quimica de Produtos Naturais e Bioativos, Universidade Estadual de Feira de Santana (UEFS), Av. Transnordestina S/N, Novo Horizonte, 44036-900, Campus Universitario, Feira de Santana, BA, Brazil.
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Alini T. Fricks*
  • Alini T. Fricks*
  • Universidade Tiradentes, Instituto de Tecnologia e Pesquisa. Laboratorio de Engenharia de Bioprocessos. Av. Murilo Dantas, 300, 49032-490, Farolandia, Aracaju, SE, Brazil.
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  •  Received: 26 November 2014
  •  Accepted: 17 March 2015
  •  Published: 25 March 2015

Abstract

Peroxidases (PODs) from radish (Raphanus sativus L.) and turnip (Brassica napus L.) were extracted and precipitated with ammonium sulfate using a simple, low cost and quick method. The activities of all steps performed by the vegetable PODs were measured via guaiacol assay. The epoxidation of isosafrol, catalyzed by radish (R. sativus L.) and turnip (B. napus L.) peroxidases was conducted in 20% (v/v) aqueous ethanol solution using 30% (v/v) H2O2 as the terminal oxidant. High conversion (88%) and selectivity (>98%) were obtained after 48 h. The products of the reaction were analyzed by high resolution gas chromatography (GC) and mass spectrometry.
 
Key words: Raphanus sativus L, Brassica napus L., peroxidase, epoxidation, isosafrol.
 

Abbreviation

HRP, Horseradish peroxidase; GC, gas chromatography; POD, peroxidases.