African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12191

Full Length Research Paper

Optimization of alkaline protease production and its fibrinolytic activity from the bacterium Pseudomonas fluorescens isolated from fish waste discharged soil

Jothiprakasam Vinoth*,
  • Jothiprakasam Vinoth*,
  • CAS in Marine Biology, Annamalai University, Parangipettai-608 502, Tamil Nadu, India.
  • Google Scholar
Sambantham Murugan
  • Sambantham Murugan
  • CAS in Marine Biology, Annamalai University, Parangipettai-608 502, Tamil Nadu, India.
  • Google Scholar
Chinnathambi Stalin
  • Chinnathambi Stalin
  • CAS in Marine Biology, Annamalai University, Parangipettai-608 502, Tamil Nadu, India.
  • Google Scholar


  •  Received: 16 April 2014
  •  Accepted: 04 July 2014
  •  Published: 23 July 2014

Abstract

Pseudomonas fluorescens AU17 was isolated from the fish waste discharged soil and it was tested for its ability to produce the protease enzyme. The effect of different production parameters such as temperature, pH, carbon and nitrogen sources and sodium chloride concentration for protease production by the isolated bacterial strain were studied. The optimum conditions observed for protease production were temperature (37°C) and pH 9, 1% wheat bran for carbon source, 0.5% peptone for nitrogen source; magnesium sulphate has less inhibitory effect among the metal ions tested. Under optimized parameters, maximum protease activity was 0.9343 U/ml/min. The bacterial isolate has potential that could be commercially exploited to assist in protein degradation in various industrial processes.

 

Key words: Alkaline protease, casein agar, fish contaminated soil, Pseudomonas fluorescens, fibrinolytic activity.