African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Cloning and characterization of functional keratin-associated protein 5-4 gene in maize

  Lin Yang, Feng-Ling Fu, Long-Qun Deng, Shu-Feng Zhou, Tai-Ming Yong and Wan-Chen Li*  
Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China.
Email: [email protected]

  •  Accepted: 02 March 2012
  •  Published: 10 April 2012

Abstract

 

Keratin-associated proteins (KAPs) 5-4 which belongs to keratin-associated protein (KRTAP) type 5 family has two major groups: high/ultrahigh cysteine (HS) and high glycine-tyrosine (HGT). Based on bioinformatic prediction, we experimentally cloned a fragment containing an open reading frame of 1849 bp from maize, which encodes a protein of 408 amino acids. BLAST analysis indicated that KAP5-4 is homologous to the qPE9-1 protein in rice. Conserved domains analysis predicted the presence of five domains. Real time reverse transcriptase polymerase chain reaction (RT-PCR) indicated that the expression of this gene is up-regulated in response to water-deficit stress in the root and leaf.

 

Key words: Maize, keratin-associated protein 5-4, water-deficit stress.

Abbreviation

Abbreviations: KAP, Keratin-associated protein; KRTAP, keratin-associated protein;HS, high/ultrahigh cysteine; HGT, high glycine-tyrosine.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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