Abstract

The purpose of this study is to produce recombinant StNOA1 in transgenic plants and to test its potential role in plant salt stress responses. The newly clonedStNOA1 gene from Solanum tuberosum L. was inserted into AtnOA1 mutant plant genome by Agrobaterium-mediated floral dip method. Transgene integration was verified by polymerase chain reaction (PCR) in 4 different lines of transgenicAtnoa1. Expression of StNOA1 gene was further analyzed by reverse trancription (RT)-PCR. Physiological analyses indicated that the transgenic line TL9 had higher proline, soluble protein and chlorophyll contents as well as lower content of malondialdehyde (MDA) compared to its receptor, Atnoa1 mutant, under salt stress condition. Root elongation and survival rate in TL9 were significantly higher than those in Atnoa1 seedlings under salt stress. Present study proved that StNOA1participated in Arabidopsis thaliana salt stress responses and increased its salinity tolerance.

Key words: StNOA1 transformation, Solanum tuberosum, Atnoa1 mutant, salt tolerance.

Abbreviation

RT-PCR, Reverse trancription-polymerase chain reaction; NO,nitric oxide; NOS, nitric oxide synthase; NR, nitrite reductase; RACE, rapid amplification of cDNA ends; TCA, trichloroacetic acid; TBA, thiobarbituric acid.