African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12196

Full Length Research Paper

Significance of fluorescent inter simple sequence repeat technique for distinguishing domestic animal breeds

Vittorio Farina
  • Vittorio Farina
  • Dipartimento di Medicina Veterinaria, Università di Sassari, Sassari, Italy.
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Gian Luca Dedola
  • Gian Luca Dedola
  • Dipartimento di Medicina Veterinaria, Università di Sassari, Sassari, Italy.
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Casmir Igbokwe
  • Casmir Igbokwe
  • Department of Veterinary Anatomy, University of Nigeria, Nsukka, Enugu State, Nigeria.
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Renato Robledo
  • Renato Robledo
  • Dipartimento di Scienze Biomediche, Università di Cagliari, Cagliari, Italy.
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Marco Casu
  • Marco Casu
  • Dipartimento di Scienze della Natura e del Territorio, Università di Sassari, Sassari, Italy.
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Daria Sanna
  • Daria Sanna
  • Dipartimento di Scienze della Natura e del Territorio, Università di Sassari, Sassari, Italy.
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Marcella Carcupino
  • Marcella Carcupino
  • Dipartimento di Scienze della Natura e del Territorio, Università di Sassari, Sassari, Italy.
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Michele Pazzola
  • Michele Pazzola
  • Dipartimento di Medicina Veterinaria, Università di Sassari, Sassari, Italy.
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Emanuela Pira
  • Emanuela Pira
  • Dipartimento di Medicina Veterinaria, Università di Sassari, Sassari, Italy.
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Giovanni Paolo Biggio
  • Giovanni Paolo Biggio
  • Dipartimento di Medicina Veterinaria, Università di Sassari, Sassari, Italy.
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Raffaele Cherchi
  • Raffaele Cherchi
  • Dipartimento di Ricerca per l'Incremento Ippico, Regione Autonoma della Sardegna, Ozieri, Italy.
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Marco Zedda
  • Marco Zedda
  • Dipartimento di Medicina Veterinaria, Università di Sassari, Sassari, Italy.
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  •  Received: 26 April 2017
  •  Accepted: 06 July 2017
  •  Published: 19 July 2017

Abstract

Conservation of farm animal genetic resources is of fundamental importance for the study of the relationships among breeds. The aim of this study was to evaluate the usefulness of the nuclear fluorescence inter simple sequence repeat (FISSR) markers in order to shed light on the genetic biodiversity of domestic animals. Two modifications of the original technique were made so as to make it more suitable for routine needs. The modified FISSR protocol was tested on different breeds of goat and donkey from Sardinia, a Mediteranean island known for its biodiversity. The two species are affected by different management problems in Sardinia: goats need a traceability of local products from different breeds, whereas donkeys are drastically reduced in number. The primers used were found to be very informative suggesting that the modified FISSR can be successfully applied in studies on different breeds of animal species without expensive experimentations. This method could be of interest in many geographic regions where there are more breeds of the same species with similar morphological features and different genetic pattern. The strongest point of this method is its low cost.

Key words: Fluorescence inter simple sequence repeat (FISSR), biodiversity, genetic variability, goat, donkey.