African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Molecular characterization of Trichoderma sp. isolated from rhizospheric soils of Uttar Pradesh (India) based on microsatellite profiles

Mohammad Shahid*
  • Mohammad Shahid*
  • Biocontrol Laboratory, Department of Plant Pathology, C. S. Azad University of Agriculture & Technology, Kanpur, U.P., India.
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Mukesh Srivastava
  • Mukesh Srivastava
  • Biocontrol Laboratory, Department of Plant Pathology, C. S. Azad University of Agriculture & Technology, Kanpur, U.P., India.
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Sonika Pandey
  • Sonika Pandey
  • Biocontrol Laboratory, Department of Plant Pathology, C. S. Azad University of Agriculture & Technology, Kanpur, U.P., India.
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Anuradha Singh
  • Anuradha Singh
  • Biocontrol Laboratory, Department of Plant Pathology, C. S. Azad University of Agriculture & Technology, Kanpur, U.P., India.
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Vipul Kumar
  • Vipul Kumar
  • Biocontrol Laboratory, Department of Plant Pathology, C. S. Azad University of Agriculture & Technology, Kanpur, U.P., India.
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  •  Received: 17 June 2014
  •  Accepted: 15 August 2014
  •  Published: 03 September 2014

Abstract

The objectives of this research were to characterize isolates of Trichoderma collected from rhizospheres of chickpea, pigeonpea and lentil crop from different places of Uttar Pradesh, India, using microsatellite-primed polymerase chain reaction (MP-PCR) and ribosomal DNA (rDNA) sequence analysis and to combine these results with morphological characteristics for clas­sification. Thirty isolates of Trichoderma sp. obtained from rhizosphere soil of plantation crops, and agricultural fields of UP region were studied using inter-simple sequence repeat (ISSR) and Internal transcribed spacer- polymerase chain reaction (ITS-PCR). The genetic relatedness among 15 isolates of Trichoderma sp. was analyzed with six micro-satellite primers. ISSR profiles showed 83.7% genetic diversity among the isolates with the formation of four clusters. Analysis of dendrogram revealed that similarity coefficient ranged from 0.27 to 0.95. ITS-PCR of rDNA region with ITS1 and ITS4 primers produced 600 bp products in all isolates. This result presented the identification patterns of Trichoderma isolates.

 

Key words: Trichoderma sp., genetic diversity, polymerase chain reaction (PCR), molecular marker, microsatellite.