African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12213

Table of Content: 3 September, 2014; 13(36)

September 2014

Genetic diversity study of common bean (Phaseolus vulgaris L.) germplasm from Ethiopia using inter simple sequence repeat (ISSR) markers

Phaseolus vulgaris L. (family Leguminosae), is a leguminous crop widely distributed in all parts of the world. In Ethiopia, common bean is cultivated as a source of protein for local consumption and for export. Mostly, it grows in the warm and lowland areas of the country. The aim of this research was to investigate the genetic diversity of P. vulgaris accessions from Ethiopia. Twelve accessions of P. vulgaris were used...

Author(s): Kefyalew Dagnew, Teklehaimanot Haileselassie and Tileye Feyissa

September 2014

Molecular characterization of Trichoderma sp. isolated from rhizospheric soils of Uttar Pradesh (India) based on microsatellite profiles

The objectives of this research were to characterize isolates of Trichoderma collected from rhizospheres of chickpea, pigeonpea and lentil crop from different places of Uttar Pradesh, India, using microsatellite-primed polymerase chain reaction (MP-PCR) and ribosomal DNA (rDNA) sequence analysis and to combine these results with morphological characteristics for clas­sification. Thirty isolates of Trichoderma sp....

Author(s): Mohammad Shahid, Mukesh Srivastava, Sonika Pandey, Anuradha Singh and Vipul Kumar

September 2014

Evaluation of different methods to overcome in vitro seed dormancy from yellow passion fruit

Seeds from yellow passion fruit (Passiflora edulis Sims) present dormancy imposed by the seed-coat. The present study aimed to evaluate some methods to overcome dormancy of seeds from P. edulis grown under in vitro conditions. The experimental design was completely randomized in factorial scheme (15 scarification methods × 2 substrates: PlantMax® and MS medium), constituting 30 treatments with 10 replicates....

Author(s): Rego, M. M., Rego, E. R., Nattrodt, L. P. U., Barroso, P. A., Finger, F. L. and Otoni, W. C.

September 2014

Canonical correlations in elephant grass for energy purposes

Elephant grass has the potential to be used as a source for energy production. Besides dry matter yield, other characteristics related to biomass quality are important. The canonic correlation analysis is a multivariate statistical procedure that allows for discovering characteristic associations among groups. The objective of this study was to evaluate the canonic correlation existing between the groups of agronomic...

Author(s): Drieli Aparecida Rossi, Bruna Rafaela da Silva Menezes, Rogério Figueiredo Daher, Geraldo de Amaral Gravina, Roberta Samara Nunes de Lima, Francisco José da Silva Lédo, Romildo Domingos Gottardo, Eliemar Campostrini and Cláudio Luiz Melo de Souza

September 2014

Evaluation of somatic embryogenesis and plant regeneration in tissue culture of ten sorghum (Sorghum bicolor L.) genotypes

Optimization of tissue culture conditions for Sorghum bicolor L. through somatic embryogenesis from immature embryos is important for the genetic manipulation and improvement of this agronomically valuable crop. In an attempt to develop a successfully reproducible in vitro regeneration protocol for a group of diverse sorghum genotypes, 10 sorghum lines including locally adapted and commercially important elite genotypes...

Author(s): Shireen Kamal Assem, Mohamed Mahmoud Zamzam, Basita Abbas Hussein and Ebtissam Hussein Aly Hussein

September 2014

Micropropagation of Plectranthus edulis (Vatke) Agnew from meristem cultur

Plectranthus edulis (Vatke) Agnew is a tuber-bearing food crop in Ethiopia. However, its productivity is hampered by shortage of pathogen-free planting materials. Therefore, the objective of this study was to develop micropropagation protocol for this plant using meristem to produce clean planting materials. Mersitems were collected from apical and axillary shoots from Holeta and Welayta areas and cultured on MS medium...

Author(s): Mesfin Tsegaw and Tileye Feyissa,

September 2014

Technique for en-masse cryo-fixation and processing of second-stage juveniles of Meloidogyne incognita for scanning electron microscopy

Large number of second-stage juveniles of root-knot nematode, Meloidogyne incognita was prepared for scanning electron microscopy over a millipore filter (pore size 0.45 µm). The J2 juveniles were quickly immobilized and killed before fixation using the cryo-fixation technique followed by the primary fixation in 2.5% glutaraldehyde pre-cooled at 4-8°C and then secondary vapor fixation in 1% osmium tetraoxide...

Author(s): Shikha Sharma, Rajdeep K. Sidhu and V. R. Parshad

September 2014

In vitro evaluation of roots, seeds and leaves of Sesamum indicum L. for their potential antibacterial and antioxidant properties

It is important to exemplify different types of medicinal plants for their potential antioxidant and antimicrobial properties as it has been evident that diverse nutrient and non-nutrient molecules which are produced from aromatic and medicinal plants display antioxidant and antimicrobial properties, and can protect human body against cellular oxidation reaction and pathogens. Methanol and aqueous extracts of different...

Author(s): Sandhya Sharma, Priti Gupta, Ashwani Kumar, Jaswant Ray, Bipin Kumar Aggarwal, Pankaj Goyal and Anubhuti Sharma,

September 2014

Statistical optimization of rapid production of cellulases from Aspergillus niger MA1 and its application in bioethanol production from rice hulls

The aim of this study was the utilization of rice hulls (RH) as a clean, cost effective, renewable and abundant agro-waste in bioethanol production by simple and applicable methods; for this purpose Aspergillus niger MA1 was isolated from RH and selected from many other isolates. This isolate produced 15.0, 3.38 and 49.1 U/g RH for CMCase, FPase and β-glucosidase, respectively, by solid state fermentation of RH...

Author(s): Mohammad M. El-Metwally, Amal M. El-Sharkawy, Adel A. El-Morsy and Salah M. El-Dohlob

September 2014

Bioethanol production from Curcubita pepo and Opilia amentacea fruits using four strains of Saccharomyces cerevisiae

Ethanol is commercially produced by fermenting agricultural products that contain high sugar or starch contents. In this study, the juices of Opilia amentacea and Curcubita pepo were used to produce bioethanol. A mixture of Urea (CON2H4), monosodium glutamate (C5H8NNaO4), potassium dihydrogen phosphate (KH2PO4), and magnesium sulfate heptahydrate (MgSO4.7H2O) is used to promote batch ethanol production with four strains...

Author(s): Kosi Mawuéna NOVIDZRO, Kokou Agbékonyi AGBODAN, Oudjaniyobi SIMALOU and Kossi Honoré KOUMAGLO

September 2014

Effect of various concentrations of Aloe vera coating on postharvest quality and shelf life of mango (Mangifera indica L.) fruits Var. ‘Ngowe’

Mango (Mangifera indica L.) is a popular and economically important tropical fruit throughout the world due to its excellent nutritional composition, eating and visual qualities. However, the fruit is highly perishable and as a result high post-harvest losses continue to be reported especially in Africa. In order to address this problem, four concentrations of Aloe vera (AG) (0, 25, 50 and 75%) and chitosan (1%) were...

Author(s): Ochiki, Sophia, Wolukau, Joseph Ngwela and Gesimba, Morwani Robert

September 2014

Seed protein electrophoresis of some members of the family fabaceae

Seed storage protein profiles of 10 members of the family Fabaceae were assessed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Total seed storage protein of the studied plants resolved on 10% SDS polyacrylamide gels showed variations in their banding pattern. Results of SDS-PAGE pattern also revealed a band common to all the plant species studied which suggests that this band could be...

Author(s): Alege, G. O, Abu Ngozi E. and Sunday, C. E