Full Length Research Paper
Abstract
An efficient protocol for Agrobacterium-mediated transformation of Serbian potato cultivars DragaÄevka and Jelica, enabling the introduction of oryzacystatin genesOCI and OCII, was established. Starting with leaf explants, a two-stage transformation protocol combining procedures of Webb and Wenzler provided high shoot regeneration efficiency: 84 - 89% for DragaÄevka cultivar and 60 - 68% for Jelica cultivar as compared to 76 - 86% for Desiree, the most frequently used cultivar in transformation experiments. PCR analysis of a small sample of putative transformants showed a nptII integration frequency of 90.9, 76.9 and 86.4% for DragaÄevka, Jelica and Desiree, respectively. Regeneration and transformation efficiency was strongly genotype-dependent.
Key words: Agrobacterium tumefaciens, oryzacystatin, Solanum tuberosum L.
Abbreviation
MS, Murashige and Skoog; OCI, oryzacystatin I; OCII,oryzacystatin II; CIM, callus-induction medium; SIM, shoot-induction medium; BA,benzylaminopurine; NAA, naphthalene acetic acid; GA3, gibberellic acid; 2,4-D, 2,4-dichlorophenoxyacetic acid; kinetin, N6-furfuryladenine.
Copyright © 2024 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0
