African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Study on optimal biodegradation of terephthalic acid by an isolated Pseudomonas sp.

Zhi-Jiang Wang1*, Li-hua Teng1, Jie-Zhang1, Xu-Li Huang1 and Jian-Fen Zhang2
1College of Biological and Environmental Science, Zhejiang Wanli University, Ningbo 315100, People’s Republic of China. 2College of Biological and Environmental Engineering, Zhejiang Shuren University, Hanzhou 310015, People’s Republic of China.
Email: [email protected]

  •  Accepted: 14 March 2011
  •  Published: 11 April 2011

Abstract

The biodegradation of terephthalic acid (TA) by the Pseudomonas sp. was researched in this paper. The optimal nitrogen source was urea with its concentration of 1400 mg/l. The optimal conditions for TA degradation were optimized by orthogonal test. The test result showed the pH 7.0, temperature of 30°C and shaking speed of 140 rpm were the best condition for degradation. In that condition, terephthalic acid was almost degraded completely after 48 h cultivation ofPseudomonas sp. by inoculating 4% seed culture. Kinetics of degradation was performed at different initial TA concentrations. The degradation could be described with a first-order kinetics model. The half-life of degradation was about 12 h when the concentration of terephthalic acid was between 600 and 1000 mg/l.

 

Key words: Biodegradation, terephthalic acid, Pseudomonas sp

Abbreviation

TA, Terephthalic acid; CFU, colony forming unit.