Full Length Research Paper
Abstract
Expression of the gene, OsAPX4, coding for ascorbate peroxidase in leaves and roots of rice were induced by abiotic stresses, such as NaCl, NaHCO3 and Na2CO3, polyethylene glycol (PEG) 6000, H2O2, CuCl2. Yeast (Saccharomyces cerevisiae) over-expressing ascorbate peroxidase exhibited greater tolerance to NaCl and NaHCO3 and transgenic Arabidopsis over-expressing OsAPX4 had a greater salt tolerance than wild-type plants in 1/2 Murashige and Skoog (MS) medium with 150, 200 mM NaCl and 5, 7.5 mM NaHCO3. These results suggest that OsAPX4 plays an important role in multiple environmental stresses.
Key words: Arabidopsis, Oryza sativa, thaliana, carbonic anhydrase ascorbate peroxides, gene expression stress.
Abbreviation
APX, Ascorbate peroxidase; ROS, reactive oxygen species;OsAPX, Oryza sativa ascorbate peroxidase gene; PEG, polyethylene glycol; MS,Murashige and Skoog; PCR, polymerase chain reaction; DIG, digoxigenin; WT,wild-type.
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