African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Genetic linkage maps of Pinus koraiensis Sieb. et Zucc. based on AFLP markers

Min-Min Chen1,3, Fujuan Feng2, Xin Sui1 and Shijie Han1*
1Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. 2Faculty of Life Science, Northeast Forestry University, Harbin 150040, China. 3Academy of Sciences, Graduate University of Chinese, Beijing 100049, China.
Email: [email protected]

  •  Accepted: 18 June 2010
  •  Published: 31 August 2010

Abstract

Genetic linkage maps provide essential information for molecular breeding. In this paper, the genetic linkage map of Pinus koraiensis was constructed using an F1 progeny of 88 individuals. One hundred and thirty (130) of molecular markers were mapped onto 6 linkage groups, 4 triples and 15 pairs at the linkage criteria LOD 4.0. Nine primer combinations were applied to map construction. The consensus map gained covers 620.909 cM, with an average marker spacing of 4.776 cM. The presented map provides crucial information for future genomic studies of P. koraiensis, in particular for QTL (quantitative trait loci) mapping of economically important breeding target traits.

 

Key words: Genetic mapping, Korean pine, linkage map, marker-aided selection.