African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Ectopic expression of a vesicle trafficking gene, OsRab7, from Oryza sativa, confers tolerance to several abiotic stresses in Escherichia coli

Xiaojue Peng, Xin Zeng, Xia Ding, Shaobo Li, Chao Yu and Youlin Zhu*
Key Laboratory of Molecular Biology and Gene Engineering, College of Life Science, Nanchang University, Nanchang 330047, P. R. China.
Email: [email protected]

  •  Accepted: 09 June 2011
  •  Published: 18 July 2011

Abstract

Rab7 is a small GTP-binding protein involved in intracellular vesicle trafficking from late endosome to the vacuole. In this study, the gene OsRab7 was isolated fromOryza sativa. Over-expression of OsRab7 gene in Escherichia coli increased the resistance to heat, cold and salt stress. In addition, subcellular localization of OsRab7 protein in E. coli and onion cells all revealed that OsRab7 protein is specifically present in the cytoplasm. These results showed that OsRab7 plays an important role in stress tolerance and probably develop abiotic stress tolerance inE. coli and planta with similar mechanism.

 

Key words: Vesicle trafficking, rice, OsRab7, Escherichia coli, abiotic stresses.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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