African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12485

Full Length Research Paper

Inhibitory activity and organic acid concentrations of metabolite combinations produced by various strains of Lactobacillus plantarum

Tran Van Thu1, Hooi Ling Foo2,3*, Teck Chwen Loh4 and Mohd Hair Bejo5
  1Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. 2Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. 3Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. 4Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. 5Department of Veterinary Pathology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Email: [email protected]

  •  Accepted: 03 January 2011
  •  Published: 21 February 2011

Abstract

 

Metabolite combinations produced by Lacrtobacillus plantarum strains were studied by inhibitory activity test using Pediococcus acidilactici as indicator microorganism. The pH and cell population of L. plantarum strains were also determined. L. plantarum RG14 strain has significant (P < 0.05) lower pH but higher cell count than the other four L. plantarum strains. Inhibitory activity of metabolite combination was stronger than single strain metabolite. However, there was significantly higher (P < 0.05) inhibitory activity in Com 2 (TL1, RG11 and RI11 strains), Com 5 (TL1, RG14 and RS5 strains) and Com 7 (RG11, RG14 and RI11 strains) than other 7 metabolite combinations, which was attributed to the present of bacteriocin inhibitory compound. Lactic and acetic acids were also present in high amount in metabolite combinations. Therefore, metabolites produced by L. plantarum strains containing mainly organic acids and bacteriocin possess vast potential as feed additives for animals.

 

Key words: Lactobacillus plantarum, metabolite combination, inhibitory activity, lactic acid, acetic acid

Abbreviation

LAB, Lactic acid bacteria; CFS, cell free supernatant; VFA, volatile fatty acids; ENT, entrobacteriaceae; VRE, vancomycin resistant enterococci.