Abstract

Gymnema sylvestre, a well known antidiabetic plant, was cultured in vitro for callus induction using leaf explant. Gymnemic acid content enhancement under the influence of different plant hormones like 2,4-D, BA with or without NaCl supplementation to the MS medium is presented in this investigation. The highest efficiency of callus formation was observed in the medium containing different concentrations of 2,4-D. The gymnemic acid content increased with increasing concentration of 2,4-D along with NaCl.

Key words: In vitro, Gymnema sylvestre, callus culture, gymnemic acid, salt stress.

Abbreviation

2, 4-D, 2, 4-Dichlorophenoxyacetic acid; BA, N6-benzylaminopurine; PGR, plant growth regulators; MS, Murashige and Skoog; Kn,kinetin; HPTLC, high performance thin layer chromatography.