African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Genetic diversity in Nigerian brinjal eggplant (Solanum melongena L.) as revealed by random amplified polymorphic DNA (RAPD) markers

Sifau, Mutiu Oyekunle*
  • Sifau, Mutiu Oyekunle*
  • Department of Cell Biology and Genetics, University of Lagos, Lagos, Lagos State, Nigeria; Molecular Biology Laboratory, Biotechnology Unit, National Centre for Genetic Resources and Biotechnology (NACGRAB), PMB 5382, Ibadan, Oyo State, Nigeria.
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Akinpelu, Adejoke
  • Akinpelu, Adejoke
  • Department of Cell Biology and Genetics, University of Lagos, Lagos, Lagos State, Nigeria.
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Ogunkanmi, Liasu Adebayo
  • Ogunkanmi, Liasu Adebayo
  • Department of Cell Biology and Genetics, University of Lagos, Lagos, Lagos State, Nigeria.
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Adekoya, Khalid Olajide
  • Adekoya, Khalid Olajide
  • Department of Cell Biology and Genetics, University of Lagos, Lagos, Lagos State, Nigeria.
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Oboh, Bola Olufunmilayo
  • Oboh, Bola Olufunmilayo
  • Department of Cell Biology and Genetics, University of Lagos, Lagos, Lagos State, Nigeria.
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Ogundipe, Oluwatoyin Temitayo
  • Ogundipe, Oluwatoyin Temitayo
  • Department of Botany, University of Lagos, Lagos, Lagos State, Nigeria.
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  •  Received: 21 November 2013
  •  Accepted: 08 May 2014
  •  Published: 21 May 2014

Abstract

The taxonomy of Solanum melongena L., also known as brinjal eggplant, has remained difficult because previous studies to establish genetic relationships among taxa are mainly based on morphological features, which are insufficient to establish genetic affinities. In the present investigation, five highly polymorphic random amplified polymorphic DNA primers were used to describe the genetic similarity and diversity among its accessions in Nigeria. The results show a high level of polymorphism based on the banding patterns among the samples. This indicated a wide and diverse genetic base. Four distinct clusters were equally noticeable at a coefficient of 0.80 from the dendrogram generated. Generally, the grouping pattern clearly indicates that irrespective of variations in fruit traits among samples, they were still grouped together in different clusters with a high similarity coefficient value. This probably showed some genetic relatedness/closeness among the samples concerned. The study also revealed that there is no association between RAPD pattern and the geographic origin of accessions. These agreed with previously published data on the characterization of eggplant. The study disclosed that molecular methods coupled with morphological analyses could make proper classification of S. melongena and other Solanum species in Nigeria possible to achieve.

 

Key words: Eggplant, random amplified polymorphic DNA (RAPD), Data, polymorphism, taxonomy.