African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Table of Content: 21 May, 2014; 13(21)

May 2014

Dearth of full-length HIV-1 sequences obscures the true HIV-1 genetic subtypes distribution in sub-Saharan Africa

HIV infection is still a public health problem in sub-Saharan Africa. The broad diversity exhibited by HIV-1 may impact on transmission, disease progression, drug resistance and vaccine development. Most analyses of HIV-1 subtype distribution have been on partial HIV-1 gene sequences, which may not adequately reflect the circulating subtypes. The objective of this study was to estimate the HIV-1 subtype distribution in...

Author(s): Kemebradikumo Pondei, Abdulrasheed Abdu and Langley Orutugu

May 2014

Chemical composition and larvicidal activity of Zanthoxylum gilletii essential oil against Anopheles gambiae

Malaria is a serious health problem in many African countries. The Anopheles gambiae mosquito which is the major vector for this disease has developed resistance against synthetic pyrethroids which are the main stay of insecticide treated bed nets. The development of insecticide resistance and side effects associated with synthetic pesticides has triggered intense research efforts towards natural products (for vector...

Author(s): Ombito O. Japheth, Matasyoh C. Josphat and Vulule M. John

May 2014

Co-feeding strategy to enhance phytase production in Pichia pastoris

Process techniques of the methylotrophic yeast Pichia pastoris for the production and recovery of heterologous phytase proteins has developed in last 20 years. High expression levels using methanol as induction have been made in the quality of recombinant proteins in the fermenter culture and in the quality of the protein product. This allowed rapidly P. pastoris to become the system of choice for the expression of...

Author(s): Ndayambaje, Jean Bernard and S. Meenakshisundaram

May 2014

Current trends in genetic manipulations to enhance abiotic and biotic stresses in tobacco

Environmental stresses, both abiotic and biotic, are the main factors reducing crops productivity. Biotic stresses caused by fungi, viruses, bacteria and insects are the major threats. However, abiotic stresses have more adverse effects on crop yield and crop survival. Hitherto, tolerant plants were mainly produced by classical breeding techniques. Success in breeding for better adapted varieties to abiotic and biotic...

Author(s): Asmamaw Tesfaw and Tileye Feyissa

May 2014

BengaSaVex: A new computational genetic sequence extraction tool for DNA repeats

The scourge of infectious diseases is one of the problems contending with humanity. All infectious diseases are caused by pathogens. A major problem in biological research is the creation of enormous and redundant amounts of genomic data. From this large volume of generated data, biologists select a subset of each sequence known as DNA nucleotide subsequences “words”, for extended scientific analysis....

Author(s): OLUWAGBEMI, Oluseun Olugbenga, IMOLORHE, Samuel and AGOZIE, Victor Okechukwu

May 2014

Sensitive and rapid detection of Mycoplasma capricolum subsp. capripneumoniae by Loop-mediated isothermal amplification

A set of four specific primers was designed by targeting the H2 gene sequences of Mycoplasma capricolum subsp. capripneumoniae (MCCP). Using Bst DNA polymerase, the products were amplified for 60 min at 65°C in a simple water bath. Compared with a polymerase chain reaction (PCR) test that targets the H2 gene sequences of MCCP, the sensitivity of the loop-mediated isothermal amplification (LAMP) assay was higher...

Author(s): HE Ying, ZHANG Nian-zhang, ZHAO Ping, CHU Yue-feng, GAO Peng-cheng, ZHANG Jian-jun, LIU Xiang-tao and Lu Zhong-xin

May 2014

Genetic diversity in Nigerian brinjal eggplant (Solanum melongena L.) as revealed by random amplified polymorphic DNA (RAPD) markers

The taxonomy of Solanum melongena L., also known as brinjal eggplant, has remained difficult because previous studies to establish genetic relationships among taxa are mainly based on morphological features, which are insufficient to establish genetic affinities. In the present investigation, five highly polymorphic random amplified polymorphic DNA primers were used to describe the genetic similarity and diversity among...

Author(s): Sifau, Mutiu Oyekunle, Akinpelu, Adejoke, Ogunkanmi, Liasu Adebayo, Adekoya, Khalid Olajide, Oboh, Bola Olufunmilayo and Ogundipe, Oluwatoyin Temitayo 

May 2014

Verification of genetic identity of introduced cacao germplasm in Ghana using single nucleotide polymorphism (SNP) markers

Accurate identification of individual genotypes is important for cacao (Theobroma cacao L.) breeding, germplasm conservation and seed propagation. The development of single nucleotide polymorphism (SNP) markers in cacao offers an effective way to use a high-throughput genotyping system for cacao genotype verification. In the present study, high-throughput genotyping with SNP markers was used to fingerprint 160 cacao...

Author(s): Jemmy Takrama, Ji Kun, Lyndel Meinhardt, Sue Mischke, Stephen Y. Opoku, Francis K. Padi and Dapeng Zhang

May 2014

Characterization and in vitro studies on anticancer activity of exopolymer of Bacillus thuringiensis S13

The objective of the present work was to isolate and characterize the exopolymer producer from a marine environment. The exopolymer producing strain (S13) was identified as Bacillus thuringiensis S13. Characterization of exopolymer shows the presence of brominated compound responsible for cytotoxicity on lung cancer cell line (A549) on XTT assay. An in vitro study of bacterial exopolymer shows the presence of cytotoxic...

Author(s): K. Parthiban, V. Vignesh and R. Thirumurugan

May 2014

Amylase production under solid state fermentation by a bacterial isolate W74

This study was concerned with the screening of a suitable isolate and optimization of cultural conditions for the biosynthesis of thermostable amylase under solid state fermentation (SSF). Twenty seven isolates were screened for amylase production out of which one isolate designated as W74 showed maximal amylase activity at 70°C and a pH of 6.5 and selected for further optimization of cultural conditions under SSF....

Author(s): Kindu Nibret Tsegaye and Amare Gessesse

May 2014

Production and partial purification of glucoamylase from Aspergillus niger isolated from cassava peel soil in Nigeria

Glucoamylase is an enzyme that hydrolyses 1,4α and 1,6β-glucosidic linkages in polysaccharides yielding glucose. Aspergillus niger strains 1, 2 and 3 were locally isolated from cassava peel dumpsite for the production of glucoamylase enzyme. A. niger strains 1, 2 and 3 were screened for their hyper producing ability on potato dextrose agar using plate assay method fortified with starch agar, and showed zone...

Author(s): Lawal, A. K., Banjoko, A. M., Olatope, S. O., Alebiosu, F. A., Orji, F. A., Suberu, Y. L., Itoandon, E. E., Shittu, K. A., Adelaja, O. D., Ojo, E., Dike, E. N. and Elemo, G. N.

May 2014

Heterologous expression and characterization of purified partial endochitinase (ech-42) isolated from Trichoderma harzianum

Chitinase gene from Trichoderma harzianum was cloned and hetrologously over expressed in M15 Escherichia coli. The recombinant protein of 42 kDa from E. coli was purified through Ni-NTA affinity column chromatography. The purified enzyme was active over broad range of pH (2.0 to 8.0) and temperature (10 to 60°C) with the peak activity at pH 5 (0.50 μg/ml) and 20°C with enzyme activity value (0.49 μg/ml)....

Author(s): Pratibha Sharma, Manika Sharma and Mukesh Srivastava