Abstract

An endophytic bacterium, H-6, was isolated from leaves of Huperzia serrata grown in the Lushan Mountain, China. The strain was identified as Burkholderia sp. H-6 based on morphological, physiological and biochemical methods as well as on 16S rDNA analysis. This strain inhibited mycelium growth in vitro of 6 plant pathogenic fungi, especially of Phytophthora capsici, Fusarium graminearumt and Sclerotinia libertiana. In greenhouse pot experiments, soil drenches with cell densities of 10⁶, 10⁸ and 10¹⁰ CFU ml^-1 H-6 reduced significantly P. capsici, in pepper seedling by 51.7, 58.7 and 60.2%, respectively, compared to the inoculated control, 3 weeks after sowing. Growth parameters such as lengths and fresh weights of roots and shoots of P. capsici-inoculated control plants were significantly lower compared toP. capsici-inoculated and H-6-treated plants, which is an added advantage of the strain used as potential biocontrol agent in future.

Key words: Endophytic bacterium, 16S rDNA gene, antagonistic activity, Huperzia serrata.