African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12300

Full Length Research Paper

Natural occurrence of products of cryptic pathway metabolites of pharmacological importance using liquid chromatographic-mass spectrometric techniques in date palm fruit, garlic bulb and groundnuts from North-West Nigeria markets

Lateef Ariyo Adeniran
  • Lateef Ariyo Adeniran
  • Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria.
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Toba Samuel Anjorin
  • Toba Samuel Anjorin
  • Department of Crop Protection, Faculty of Agriculture, University of Abuja, PMB 117, Abuja, Nigeria.
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Michael Sulyok
  • Michael Sulyok
  • Department of Agrobiotechnology (IFA-Tulln), Institute of Bioanalytics and Agro-Metabolomics, University of Natural Resources and Life Sciences, Vienna, Konrad Lorenzstr Austria.
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Rudolf Krska
  • Rudolf Krska
  • Department of Agrobiotechnology (IFA-Tulln), Institute of Bioanalytics and Agro-Metabolomics, University of Natural Resources and Life Sciences, Vienna, Konrad Lorenzstr Austria.
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Olatunde Peter Ajagbonna
  • Olatunde Peter Ajagbonna
  • Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Abuja, Nigeria.
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  •  Received: 07 July 2020
  •  Accepted: 12 August 2020
  •  Published: 31 August 2020

Abstract

Investigation of secondary metabolites from the cryptic metabolic pathways in date palm fruit, garlic bulb and groundnuts from North-West Nigeria was conducted using liquid chromatography-mass spectrometry and mass spectrometry (LC-MS/MS). Results indicated the presence of several compounds which include Valinomycin, asperglaucide, asperphenamate, cyclo (L-Pro-L-Tyr). Cyclo (L-Pro-L-Val), emodin, physcion, integracin A and B, Monocerin, and fallacinol were detected at different concentrations in the different sampled materials. Asperglaucide, asperphenamate, cyclo (L-Pro-L-Tyr), cyclo (L-Pro-L-Val), and emodin were found in all the tested samples at different concentrations. Garlic bulb contains valinomycin, emodin and physcion which are compound that may have a potential inhibitory effect on severe acute respiratory syndrome coronavirus (SARS-CoV) with their maximum concentration of 55.15, 20.25 and 419.45 µg/kg respectively in the tested materials. Integracin A and B which are compound with potential inhibitory effect on the HIV-1 integrase enzyme found in Human immune deficiency virus (HIV) were detected in garlic bulb samples at the maximum concentration of (3.98 and 18.68 µg/kg) respectively. This study has identified the presence of a compound of immense pharmacological importance, and it may provide a veritable lead for natural product discovery.

 

Key words: Asperphenamate; cryptic pathway; date fruits; garlic bulb; groundnut seed; metabolites.