African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12483

Full Length Research Paper

Semi-artificial method of induced breeding of the African catfish (Clarias gariepinus, Burchell, 1822) under varying broodstock ratios using Ovaprim®

Addo Samuel
  • Addo Samuel
  • Department of Marine and Fisheries Sciences, University of Ghana, Legon, Ghana.
  • Google Scholar
Ameti Kennedy S.
  • Ameti Kennedy S.
  • Ohawu Agricultural College - Ohawu, Volta Region, Ghana.
  • Google Scholar
Sowah Winnie
  • Sowah Winnie
  • Department of Marine and Fisheries Sciences, University of Ghana, Legon, Ghana.
  • Google Scholar
Diyie Rhoda Lims
  • Diyie Rhoda Lims
  • Water Research Institute, CSIR, Ghana.
  • Google Scholar
Duodu Collins P.
  • Duodu Collins P.
  • Department of Marine and Fisheries Sciences, University of Ghana, Legon, Ghana.
  • Google Scholar
Asamoah Eunice K.
  • Asamoah Eunice K.
  • Department of Marine and Fisheries Sciences, University of Ghana, Legon, Ghana.
  • Google Scholar


  •  Received: 22 July 2022
  •  Accepted: 29 September 2022
  •  Published: 31 October 2022

Abstract

Modern methods of producing African catfish (Clarias gariepinus) fingerlings require that the male brooder is sacrificed to obtain milt for artificial fertilization of the eggs stripped from the female under hormonal induction. This study assessed the semi-artificial technique of producing catfish larvae with different broodstock ratios using Ovaprim®, a synthetic spawning inducing hormone. The treatments with 3 replicate each were: T1 (artificial spawning with 1:1 female: male ratio), T2 (semi-artificial spawning with 1:1 female: male ratio), and T3 (semi-artificial spawning with 2:1 female: male ratio). The relative fecundity of brooders in T1, T2, and T3 was 68 ± 6.31, 78 ± 12.29, and 65 ± 8.18, respectively with no significant difference (P ≥ 0.05). Percent fertilization for T1 (81±1.52%), T2 (75 ± 2.51%) and T3 (62 ± 2.50%) was significantly different (P ≤ 0.05). The observed percent hatchability (85 ± 2.51, 83 ± 3.21, and 82 ± 2.50%) in respect of T1, T2, and T3 was not statistically different (P ≥ 0.05). Differences in total egg weight (96 ± 3.30, 72 ± 10.53, and 59 ± 0.50 g; p=0.099), and total larval production (57,700 ± 3672; 42,423 ± 6972 and 34,078 ± 762; p=0.002) for T1, T2 and T3, respectively, were statistically significant between artificial spawning and semi-artificial spawning. Larval survival was significant (P ≤ 0.05) between T1 (84 ± 2.31) and T3 (92 ± 2.50) but both did not differ significantly (P ≥ 0.05) from T2 (87 ± 2.51). In conclusion, the semi-artificial spawning of C. gariepinus with Ovaprim® could be beneficial to fish farmers if done at a broodstock sex pairing ratio of 1:1.

 

Key words: Clarias gariepinus, induced breeding, Ovaprim®, semi-artificial, aquaculture.